Member for producing a probe array and probe array producing method

• US 20070275405A1
• Filed: 07/12/2007
• Published: 11/29/2007
• Est. Priority Date: 03/29/2004
• Status: Abandoned Application

• Current Assignee: Lintec Corporation, Riken
• Original Assignee: Lintec Corporation, Riken
• Inventors: Tashiro, Hideo, Kondoh, Yasumitsu, Yamamoto, Yuko, Okabe, Hideaki
• Application Number:
  US11/826,060
• Publication Number:
  US 20070275405A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00475 Sheets
• CPC Class Codes: B01J 2219/00511 Walls of reactor vessels
• CPC Class Codes: B01J 2219/00538 in the shape of cylinders
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries

Abstract

In order to provide a member for producing a probe array that allows producing desired probe arrays immediately once the probe arrangement is established, and to provide a probe array producing method that uses this member for producing a probe array, the pieces F₁, F₂ . . . F_n are produced by cutting the members M₁, M₂ . . . M_n for producing a probe array, and a predetermined number of pieces F₁, F₂ . . . F_n are detached from a release sheet 13 and are attached via the adhesive layer 12 onto the supporting member 2a at predetermined locations.

Claims

1-3. -3. (canceled)

4. A probe array comprising:

a supporting member; and

the member for producing a probe array or a piece thereof, arranged on the surface of the supporting member, the member including a substrate, probes immobilized on a first region of the surface of the substrate and an adhesive layer provided on a second region of the surface of the substrate;

wherein the member for producing the probe array or the piece thereof is attached via the adhesive layer on the supporting member in such a way that the probes are exposed.

5. The probe array according to claim 4, wherein the supporting member is a tubular member, and the member for producing a probe array or the piece thereof is attached on the inner face of the tubular member.

6. The probe array according to claim 4, wherein the probe array further comprises a tubular member, and the supporting member is housed in the hollow portion of the tubular member in such a way that the probes are exposed to the hollow portion of the tubular member.

7. The probe array according to claim 6, wherein a spacer for preventing contact between the outer face of the supporting member and the inner face of the tubular member is provided on the outer face of the supporting member or on the inner face of the tubular member.

13. The probe array according to claim 4, wherein the substrate is a sheet-like substrate, the first region is positioned on one face of the sheet-like substrate, and the second region is positioned on the other face of the sheet-like substrate.

14. The probe array according to claim 4, wherein only one type of probes are immobilized on the first region.

8. A method for producing a probe array comprising a supporting member, and a member for producing a probe array or a piece thereof, arranged on the surface of the supporting member, the member including a substrate, probes immobilized on a first region of the surface of the substrate, and an adhesive layer provided on a second region of the surface of the substrate the method comprising a step of attaching via the adhesive layer the member for producing a probe array or the piece thereof on the supporting member in such a way that the probes are exposed.

9. The probe array producing method according to claim 8, wherein the supporting member is a tubular member, and in the step the member for producing a probe arrays or the piece thereof is attached on the inner face of the tubular member.

10. The probe array producing method according to claim 8, wherein the probe array further comprises a tubular member, and the method further comprises a step of housing the supporting member in the tubular member in such a way that the probes are exposed to the hollow portion of the tubular member.

11. The probe array producing method according to claim 8, wherein the substrate is a sheet-like substrate, the first region is positioned on one face of the sheet-like substrate, and the second region is positioned on the other face of the sheet-like substrate.

12. The probe array producing method according to claim 8, wherein only one type of probes are immobilized on the first region.