Monoclonal Antibodies With Specificity For Fetal Erythroid Cells

• US 20070275418A1
• Filed: 03/31/2005
• Published: 11/29/2007
• Est. Priority Date: 03/31/2004
• Status: Active Grant

• Current Assignee: KellBenx Inc.
• Original Assignee: AdnaGen AG
• Inventors: Albert, Winfried, Zimmermann, Silke, Stachelhaus, Stefan, Hollmann, Christiane
• Granted Patent: US 7,858,757 B2
• Time in Patent Office: Days
• US Class Current: 435/7.210
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: C07K 2317/14 Specific host cells or culture conditions, e.g. components, pH or temperature
• CPC Class Codes: G01N 2333/70582 CD71
• CPC Class Codes: G01N 2469/10 Detection of antigens from microorganism in sample from host
• CPC Class Codes: G01N 33/5094 for blood cell populations red blood cells G01N33/80
• CPC Class Codes: G01N 33/56966 Animal cells
• CPC Class Codes: G01N 33/577 involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens; G01N33/53 - G01N33/576 take precedence
• CPC Class Codes: G01N 33/80 involving blood groups or blood types or red blood cells white blood cells G01N33/56972
• CPC Class Codes: Y10T 436/25125 Digestion or removing interfering materials
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

The present invention concerns a monoclonal antibody and corresponding hybridoma cells and antigens suitable for isolating fetal cells from maternal blood. The monoclonal antibody reacts with a surface antigen present on fetal red blood cells including their nucleated precursor cells, but not with surface antigens on adult erythroid cell.

Claims

1. A monoclonal antibody reacting with a surface antigen present on fetal red blood cells including their nucleated precursor cells, but not with surface antigens on adult erythroid cells.

2. The antibody according to claim 1, characterized in that it reacts with most or all fetal erythroid cells, which express the CD71 antigen but are negative for CD45 antigen expression.

3. The antibody according to claim 1, characterized in that it reacts with fetal erythroid cells but not with the CD71 antigen.

11. Use of a monoclonal antibody according to claim 1 for the detection and identification of fetal cells in a sample.

12. Use according to claim 11 for the detection and identification of fetal cells in a sample of maternal blood.

13. A method for detection or identification of fetal cells in a sample, characterized by labeling said fetal cells by an antibody according to claim 1.

14. The method according to claim 13, characterized in that the sample is maternal blood or a sample of maternal blood.

15. The method according to claim 13, where said fetal cells are subsequent to the detection and identification further analyzed for a chromosomal and/or genetic aberration, defects and/or variant.

16. The method according to claim 13, further comprising separating cells binding the monoclonal antibody by flow cytometry, solid phase separation, immunomagnetic bead separation, panning on plastic surfaces, or the like.

4. A hybridoma cell producing monoclonal antibody reacting with a surface antigen present on fetal red blood cells including their nucleated precursor cells, but not with surface antigens on adult erythroid cells.

5. A hybridoma cell as deposited under accession number DSM ACC 2666 on Jul. 13, 2004 at the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH in Braunschweig, Germany.

7. An antibody expressed by the hybridoma cell according to claim 5.

9. A surface antigen on fetal red blood cells recognized by a monoclonal antibody as characterized in claim 5.

6. (canceled)

8. (canceled)

10. An antibody characterized in that it recognizes or binds specifically to a surface antigen on fetal red blood cells.