Compositions and Methods for Producing Fermentation Products and Residuals
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Abstract
The present invention provides compositions and methods designed to increase value output of a fermentation reaction. In particular, the present invention provides a business method of increasing value output of a fermentation plant. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals. Further provided by the present invention is a method of performing fermentation, a modified fermentative microorganism and a genetic vehicle for modifying such microorganism.
90 Citations
103 Claims
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1-37. -37. (canceled)
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38. A method of fermentation using carbon-containing material, comprising:
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(a) mixing a carbon-containing material with a culture comprising genetically modified microorganisms that, in a fermentation reaction, produce an alcohol and a fermentation residual comprising a nutrient selected from the group consisting of amino acids, cofactors, hormones, proteins, vitamins and lipids, wherein the content of the nutrient in the fermentation residual is greater than that of an unmodified corresponding microorganism when used in the fermentation reaction;
(b) fermenting the culture under conditions suitable for production of the alcohol and under conditions suitable for production the nutrient;
(c) separating the alcohol from the culture; and
(d) producing the fermentation residual comprising the nutrient. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68)
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- 69. A genetically modified microorganism that, in a fermentation reaction, produces an alcohol and a fermentation residual comprising a nutrient selected from the group consisting of amino acids, cofactors, hormones, proteins, vitamins, and lipids, wherein the content of the nutrient in the fermentation residual is greater than that of an unmodified corresponding microorganism when used in the fermentation reaction.
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93. A fermentation culture comprising:
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(a) a genetically modified microorganism that, in a fermentation reaction, produces an alcohol and a fermentation residual comprising a nutrient selected from the group consisting of an amino acid, a cofactor, a hormone, a protein, a vitamin, and a lipid, wherein the content of the nutrient in the fermentation residual is greater than that of an unmodified corresponding microorganism when used in the fermentation reaction and (b) a fermentation medium for production of nutrients comprising a carbon source. - View Dependent Claims (94, 95, 96, 97, 98, 99, 100)
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- 101. An expression vector comprising an exogenous sequence encoding a polypeptide comprising at least one essential amino acid for a domesticated animal, wherein expression of the exogenous sequence is induced when a fermentation reaction producing an alcohol or an alkane has achieved at least about 50% completion.
Specification