Biological reaction method and bioreactor

• US 20070275452A1
• Filed: 04/11/2007
• Published: 11/29/2007
• Est. Priority Date: 05/26/2006
• Status: Active Grant

• Current Assignee: Sharp Kabushiki Kaisha (Hon Hai Precision Industry Co., Ltd.)
• Original Assignee: Sharp Kabushiki Kaisha (Hon Hai Precision Industry Co., Ltd.)
• Inventors: Yamasaki, Kazuyuki, Kataoka, Masaki, Sakata, Kazuyuki, Chuhjoh, Kazumi
• Granted Patent: US 8,043,848 B2
• Time in Patent Office: Days
• US Class Current: 435/252.100
• CPC Class Codes: B01F 2101/44 Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
• CPC Class Codes: C12M 27/02 Stirrer or mobile mixing elements
• CPC Class Codes: C12M 29/02 Percolation
• CPC Class Codes: C12M 29/06 Nozzles; Sprayers; Spargers; Diffusers per se B01F23/231, B01J19/26
• CPC Class Codes: C12M 37/02 Filters

Abstract

In a bioreactor, a culture solution derived from a cultivation tank is separated into bacteria cells and filtrate by a bacteria cell filter. The filtrate is introduced from the bacteria cell filter into a micro-nano bubble generation tank where micro-nano-bubbles are mixed with the filtrate. The filtrate containing micro-nano-bubbles is returned to the cultivation tank to activate the microorganisms therein, so that a biological reaction time is reduced by the activated microorganisms.

Claims

1. A biological reaction method, comprising the steps for:

adding micro-nano-bubbles into a culture solution; and

cultivating microorganisms in the culture solution containing the micro-nano-bubbles in a biological reaction tank.

2. A bioreactor comprising a biological reaction tank for cultivating microorganisms in a culture solution containing the micro-nano-bubbles.

3. The bioreactor set forth in claim 2, further comprising:

a seed bacteria cultivation tank for cultivating microorganisms as seed bacteria in the culture solution containing the micro-nano-bubbles and for introducing the culture solution containing the seed bacteria into the biological reaction tank.

4. The bioreactor set forth in claim 3, further comprising:

a medium reservoir for supplying a medium to the biological reaction tank;

a bacteria cell filter for receiving the culture solution from the biological reaction tank and separating the culture solution into bacteria cells and filtrate; and

a micro-nano bubble generation tank for receiving the filtrate from the bacteria cell filter, adding the micro-nano-bubbles to the filtrate, and introducing the filtrate containing the micro-nano-bubbles into at least one of the seed bacteria cultivation tank and the biological reaction tank.

5. The bioreactor set forth in claim 4, further comprising

an air supply section for supplying air disinfected with a disinfection filter to the seed bacteria cultivation tank and the biological reaction tank, and for aerating and stirring the seed bacteria cultivation tank and the biological reaction tank.

6. The bioreactor set forth in claim 4, further comprising:

a medium adjustment tank for adjusting a plurality of raw media materials; and

a sterilization section for sterilizing the raw media materials adjusted in the medium adjustment tank and introducing the sterilized raw media materials into the medium reservoir.

7. The bioreactor set forth in claim 4, further comprising:

a filtrate reservoir for receiving the filtrate from the bacteria cell filter and adding the micro-nano-bubbles to the filtrate; and

a distiller for receiving the filtrate containing the micro-nano-bubbles from the filtrate reservoir and distilling the filtrate containing the micro-nano-bubbles.

8. The bioreactor set forth in claim 7, wherein

the filtrate reservoir has a micro-nano bubble generator.

9. The bioreactor set forth in claim 4, wherein

a filler material is placed in the biological reaction tank.

10. The bioreactor set forth in claim 9, wherein

the filler material is a string-type polyvinylidene chloride filler.

11. The bioreactor set forth in claim 4, wherein

a filler material is placed in the seed bacteria cultivation tank.

12. The bioreactor set forth in claim 11, wherein

the filler material is a string-type polyvinylidene chloride filler.

13. The bioreactor set forth in claim 4, wherein

the biological reaction tank and the seed bacteria cultivation tank are filled with a filler material.

14. The bioreactor set forth in claim 13, wherein

the filler material is a string-type polyvinylidene chloride filler.