Cell Culture Surface Chemistries

• US 20070275457A1
• Filed: 05/25/2006
• Published: 11/29/2007
• Est. Priority Date: 05/25/2006
• Status: Active Grant

• Current Assignee: Nalge NUNC International Corporation (Perspecta, Inc.)
• Original Assignee: Nalge NUNC International Corporation (Perspecta, Inc.)
• Inventors: Cummins, Thomas, Granchelli, Joe
• Granted Patent: US 8,642,307 B2
• Time in Patent Office: Days
• US Class Current: 435/289.100
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12M 25/06 Plates; Walls; Drawers; Multilayer plates
• CPC Class Codes: C12N 2533/20 Small organic molecules
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A surface supporting growth of cells in culture, the surface prepare to provide hydrophilic moieties exposed thereon for enhancing the attachment of biological materials. The surface contains the hydrophilic moiety as part of an amphipathic molecule also having a hydrophobic region that is anchored or embedded within a polymer substrate, and oriented such that the hydrophilic moieties are exposed on the outer surface of the substrate. In one embodiment the surface is customized to provide qualitative and quantitative moieties to enhance particular cell growth. In one embodiment, the surface is provided on a culture apparatus such as a dish or plate.

Claims

1. An apparatus for the culture of biological materials, the apparatus comprising

a nonfibrous substrate for the attachment of biological materials, the substrate prepared from a blend of polymer plastic resin and amphipathic molecules;

wherein the amphipathic molecules comprise hydrophilic moieties and a hydrophobic region, the hydrophobic region being embedded within the substrate, and the hydrophilic moieties being exposed on the substrate.

2. The apparatus of claim 1 wherein the resin of the blend is coated with the amphipathic molecules.

3. The apparatus of claim 1 wherein the resin of the blend is compounded with the amphipathic molecules.

4. The apparatus of claim 1 wherein the hydrophilic moieties are chosen from hydroxyls, carboxyls, amines, or mixtures thereof.

5. The apparatus of claim 1 wherein the plastic polymer of the surface is chosen from polystyrene, polypropylene, glycol-modified polyethylene terephthalate (PETG), polyethylene terephthalate (PET), polycarbonate, or mixtures thereof.

6. The apparatus of claim 1 wherein the amphipathic molecules are blended with the polymer plastic resin at a proportion of about 1% w/w.

7. The apparatus of claim 1 wherein the amphipathic molecules are blended with the polymer plastic resin at a proportion of about 0.1% w/w.

8. The apparatus of claim 1 wherein the surface is further treated with corona discharge or plasma discharge.

9. The apparatus of claim 1 wherein the surface is further coated with poly-D-lysine or collagen.

10. The apparatus of claim 1 wherein the hydrophobic region is a chain of carbon linked atoms having a length between 10 to 30 carbon atoms.

11. The apparatus of claim 1 wherein the biological materials have an adherent phenotype.

12. The apparatus of claim 11 wherein the biological materials is selected from the group consisting of cells, organelles, subcellular structures, viruses, bacteria, biomolecules, and combinations thereof

13. The apparatus of claim 1 wherein the amphipathic molecules are chosen from alkylamine ethoxylate, polyethylenimine, octyldecamine, or mixtures thereof.

14. The apparatus of claim 1 wherein the amphipathic molecules do not include fluorocompounds.

15. A method of making an apparatus for the culture of biological materials, the method comprising

determining a type of hydrophilic functional group that facilitates association with a biological material of an adherent phenotype;

selecting an amphipathic molecule including hydrophilic and hydrophobic regions, the hydrophilic region including said hydrophilic functional group;

blending the amphipathic molecule with a polymer plastic resin to form a blended resin; and

preparing an apparatus for the culture of the biological material from said blended resin.

16. The method of claim 15 further comprising determining a concentration of the hydrophilic functional group that facilitates association with the biological material.

17. The method of claim 16 wherein blending the amphipathic molecule with the polymer plastic resin further comprises blending the amphipathic molecule in a concentration, relative to the polymer plastic resin, substantially similar to the concentration that facilitates association with the biological material.

18. The method of claim 15 wherein blending the amphipathic molecule with the polymer plastic resin further comprises coating the polymer plastic resin with the amphipathic molecule.

19. The method of claim 15 wherein blending the amphipathic molecule with the polymer plastic resin further comprises compounding the polymer plastic resin with the amphipathic molecule.

20. The method of claim 15 further comprising treating a surface of the cell culture apparatus with corona discharge or plasma discharge.

21. The method of claim 15 further comprising coating a surface of the cell culture apparatus with poly-D-lysine or collagen.