Assay Chip, and Uses of Said Assay Chip to Determine Molecular Structures and Functions
First Claim
1. An assay chip for investigation of a functionality of non-lipid molecules and their interactions with molecules, comprising:
- a) a nanopore substrate having a plurality of nanopores;
b) a substantially planar support layer deposited on said nanopore substrate and having a plurality of nanopores corresponding to said nanopores of said nanopore substrate;
c) a biologically effective layer configured to host at least one of a non-lipid molecule and functional molecule, deposited on said support layer and covering the plurality of nanopores, resulting in accessible nanopores from both sides of the biologically effective layer for measurements.
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Accused Products
Abstract
An assay chip for investigation the functionality of membrane proteins and their interactions with molecules includes a nanopore array having a plurality of nanopores in a suitable support layer deposited on the nanopore substrate and being a substantially planar support layer having a plurality of nanopores corresponding to the nanopores of the nanopore substrate. The chip includes further a biologically effective layer capable to host at least a non-lipid molecule or functional molecule, deposited on the support layer and covering the plurality of nanopores, resulting in accesible nanopores from both sides of the biologically effective layer for measurement or imaging. The structured support allows generating a biologically effective membrane, such as a lipid bilayer membrane, having high and reliable stability in a manner that its fluidity is sustained in order to keep integrated membrane proteins in the lipid bilayer in its full biological functionality.
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Citations
19 Claims
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1. An assay chip for investigation of a functionality of non-lipid molecules and their interactions with molecules, comprising:
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a) a nanopore substrate having a plurality of nanopores;
b) a substantially planar support layer deposited on said nanopore substrate and having a plurality of nanopores corresponding to said nanopores of said nanopore substrate;
c) a biologically effective layer configured to host at least one of a non-lipid molecule and functional molecule, deposited on said support layer and covering the plurality of nanopores, resulting in accessible nanopores from both sides of the biologically effective layer for measurements. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 17, 18, 19)
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13. A process for analyzing a functionality of at least one of a non-lipid molecule and functional molecule, being integrated in a biologically effective layer of an assay chip comprising a nanopore substrate having a plurality of nanopores, a substantially planar support layer deposited on said nanopore substrate and having a plurality of nanopores corresponding to said nanopores of said nanopore substrate, and a biologically effective layer configured, the process comprising:
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a) applying a fluid containing a binding compound to one side of the biologically effective layer in order to allow the binding compound to interact with the non-lipid molecule;
b) monitoring the response of the non-lipid molecule induced by at least one of effector binding and an interaction of binding molecules in the biologically effective layer by measuring physical or chemical changes on cis- or trans-sides of the assay chip.
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14. (canceled)
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15. (canceled)
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16. (canceled)
Specification