Compositions and Methods for Stem Cell Expansion and Differentiation
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Abstract
The present invention relates to compositions comprising stem cells and partially committed progenitor cells and to methods of controlling cell proliferation and differentiation, which can be used for expansion of stem cells and their subsequent differentiation. The present invention provides expanded population of essentially undifferentiated stem cells, which are useful in clinical procedures involving stem cell therapy, and a population derived thereof of which at least part of the cells are differentiated. The cells can be used per se, as a part of a cell-bearing composition comprising cross-linked hyaluronic acid-laminin gels or as a part of a composite implant for tissue regeneration.
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Citations
134 Claims
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1-73. -73. (canceled)
- 74. A composition for expanding stem cells, comprising a population of stem cells cultured in or on a biocompatible matrix comprising hyaluronic acid and laminin cross-linked to form a combined gel, further comprising nutrient culture medium, suitable for supporting the growth of stem cells wherein at least the majority of the cells are in their undifferentiated state.
- 83. A composition comprising a population of cells cultured in or on a biocompatible matrix comprising hyaluronic acid and laminin cross-linked to form a combined gel, further comprising nutrient culture medium, wherein the cell population is derived from stem cells, and wherein at least part of the cells are differentiated to a desired cell type.
- 92. A composite implant comprising cells cultured in or on cross-linked hyaluronic acid-laminin gel further comprising nutrient culture medium and a biocompatible scaffold, wherein the scaffold supports the cultured cells.
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105. A method for expanding stem cells, comprising:
- (a) providing a population of stem cells; and
(b) culturing the population of stem cells in or on a composition comprising biocompatible matrix comprising hyaluronic acid and laminin cross-linked to form a combined gel, further comprising nutrient culture medium, under conditions wherein the cultured cells are proliferating while maintaining their undifferentiated state. - View Dependent Claims (106, 107, 108, 109, 110, 111, 112, 113)
- (a) providing a population of stem cells; and
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114. The method according to claim 114, wherein transformation is performed prior to culturing the stem cell population in or on the hyaluronic acid and laminin combined gel or before re-seeding isolated cells during culture passages.
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115. A method for differentiating stem cells, comprising:
- (a) providing a population of stem cells; and
(b) culturing the population of stem cells in or on a composition comprising biocompatible matrix comprising hyaluronic acid and laminin cross-linked to form a combined gel, further comprising nutrient culture medium, under conditions wherein at least part of the stem cells differentiate to a desired cell type. - View Dependent Claims (116, 117, 118, 119, 120, 121, 122)
- (a) providing a population of stem cells; and
- 123. A method for transplanting cells to an individual in need thereof, comprising transplanting a composite implant comprising the cells cultured in or on cross-linked hyaluronic acid-laminin gel, and a biocompatible scaffold, wherein the scaffold supports said cultured cell.
Specification