Acousto-Mechanical Detection Systems and Methods of Use
First Claim
1. A system for detecting a target biological analyte, the system comprising:
- a surface acoustic wave sensor comprising a detection surface;
a capture agent located on the detection surface, wherein the capture agent is capable of selectively attaching the target biological analyte to the detection surface;
a detection chamber located within an interior volume of a housing, the detection chamber comprising a volume defined by the detection surface and an opposing surface spaced apart from and facing the detection surface, wherein the opposing surface of the detection chamber comprises a flow front control feature;
a waste chamber located within the interior volume of the housing, the waste chamber in fluid communication with the detection chamber;
means for driving the shear horizontal surface acoustic wave sensor;
means for analyzing data from the surface acoustic wave sensor to determine if target biological analyte is coupled to the capture agent.
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Accused Products
Abstract
Detection systems and methods for detecting target biological analytes within sample material using acousto-mechanical energy generated by a sensor are disclosed. The acousto-mechanical energy may be provided using an acousto-mechanical sensor, e.g., a surface acoustic wave sensor such as, e.g., a shear horizontal surface acoustic wave sensor (e.g., a LSH-SAW sensor). A variety of techniques for modifying the effective mass of the target biological analytes in sample material are disclosed, including fractionating or disassembling the target biological analytes in the sample material (e.g., lysing the target biological analyte), adding a detectable mass to the target biological analyte or enhancing coupling of the target biological analyte (e.g., through the use of magnetic particles), exposing the sample material to a reagent that causes a change in at least detectable physical property in the sample material if the target biological analyte is present (e.g., a change in viscous, elastic, and/or viscoelastic properties), etc.
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Citations
47 Claims
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1. A system for detecting a target biological analyte, the system comprising:
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a surface acoustic wave sensor comprising a detection surface;
a capture agent located on the detection surface, wherein the capture agent is capable of selectively attaching the target biological analyte to the detection surface;
a detection chamber located within an interior volume of a housing, the detection chamber comprising a volume defined by the detection surface and an opposing surface spaced apart from and facing the detection surface, wherein the opposing surface of the detection chamber comprises a flow front control feature;
a waste chamber located within the interior volume of the housing, the waste chamber in fluid communication with the detection chamber;
means for driving the shear horizontal surface acoustic wave sensor;
means for analyzing data from the surface acoustic wave sensor to determine if target biological analyte is coupled to the capture agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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23. A system for detecting a target biological analyte, the system comprising:
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a shear horizontal surface acoustic wave sensor comprising a detection surface;
a capture agent located on the detection surface, wherein the capture agent is capable of selectively attaching the target biological analyte to the detection surface;
a detection chamber located within an interior volume of a housing, the detection chamber comprising a volume defined by the detection surface and an opposing surface spaced from and facing the detection surface, wherein the opposing surface of the detection chamber comprises a flow control feature;
a waste chamber in fluid communication with the detection chamber, wherein absorbent material is located within the waste chamber;
capillary structure located between the detection chamber and the waste chamber;
at least one module comprising an exit port attached to the housing through a module port that opens into the interior volume of the housing, wherein the at least one module contains a selected reagent within a chamber, and further wherein the at least one module comprises an exit seal closing the exit port of the at least one module, a plunger located within the at least one module, wherein the plunger is movable from a loaded position in which the plunger is distal from the exit port to an unloaded position in which the plunger is proximate the exit port, wherein movement of the plunger towards the exit port opens the exit seal and delivers material from the chamber of the at least one module into the interior volume of the housing through the exit port;
an actuator operably coupled to the plunger of the at least one module, wherein the actuator is capable of moving the plunger from the loaded position to the unloaded position;
means for driving the shear horizontal surface acoustic wave sensor; and
means for analyzing data from the shear horizontal surface acoustic wave sensor to determine if the target biological analyte is coupled to the capture agent. - View Dependent Claims (24, 25, 26, 27, 28)
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40. A method of detecting a biological analyte, the method comprising:
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fractionating target biological analyte located within sample material;
contacting a detection surface of a shear horizontal surface acoustic wave sensor with the sample material containing the fractionated target biological analyte;
selectively attaching the fractionated target biological analyte to the detection surface; and
operating the shear horizontal surface acoustic wave sensor to detect the attached fractionated target biological analyte while the detection surface is submersed in liquid. - View Dependent Claims (41, 42, 43, 44, 45)
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46. A shear horizontal surface acoustic wave sensor comprising:
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a piezoelectric substrate comprising a major surface;
at least one transducer on the major surface of the piezoelectric substrate, wherein the at least one transducer defines an acoustic path on the major surface of the piezoelectric substrate, wherein the acoustic path comprises a first end and a second end;
wherein the at least one transducer comprises a contact pad on the major surface of the piezoelectric substrate, wherein the contact pad is located off to a first side of the acoustic path and between the first end and the second end of the acoustic path, wherein the contact pad is connected to the at least one transducer by a lead. - View Dependent Claims (47)
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Specification