Microfluidic Device

• US 20070286773A1
• Filed: 08/22/2007
• Published: 12/13/2007
• Est. Priority Date: 05/16/2002
• Status: Abandoned Application

• Current Assignee: Micronit Microfluidics B.V.
• Original Assignee: Micronit Microfluidics B.V.
• Inventors: Schlautmann, Stefan, Van den Berg, Albert, Gardeniers, Johannes Gerardus
• Application Number:
  US11/843,124
• Publication Number:
  US 20070286773A1
• Time in Patent Office: Days
• US Class Current: 422/68.100
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0418 electro-osmotic flow [EOF]
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 7/54 using spatial temperature gradients
• CPC Class Codes: B81B 2201/058 Microfluidics not provided for in B81B2201/051 - B81B2201/054
• CPC Class Codes: B81B 2203/0338 Channels
• CPC Class Codes: B81C 1/00206 Processes for functionalising a surface, e.g. provide the surface with specific mechanical, chemical or biological properties
• CPC Class Codes: B81C 2201/019 Bonding or gluing multiple substrate layers
• CPC Class Codes: B81C 3/001 Bonding of two components
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

The present invention relates to a method of fabricating a microfluidic device including at least two substrates provided with a fluid channel, comprising the steps of: a) etching at least a channel and one or more fluid ports in a first and/or a second substrate; b) depositing a first layer on a surface of the second substrate; c) partially removing the first layer in accordance with a predefined geometry; d) depositing a second layer on top of the first layer and the substrate surface; e) planarizing the second layer so as to smooth the upper surface thereof; f) aligning the first and second substrate; and g) bonding the first substrate on the planarized second layer of the second substrate.

Claims

1. A microfluidic device, comprising:

a) a first and a second substrate;

b) a fluid channel;

c) at least a channel and one or more fluid ports etched in the first and/or second substrates;

d) a first layer deposited on a surface of the second substrate, from which the first layer is partially removed in accordance with a predefined geometry; and

e) a second layer deposited on top of the first layer and the surface of the second substrate;

wherein the first substrate is aligned relative to the surface of the second substrate and thereafter bonded therewith, and

wherein the first layer is arranged relative to the channel so as to influence the transport or the properties of the fluid in the channel.

2. The microfluidic device as claimed in claim 1, wherein the second layer is planarized prior to alignment and bonding of the first substrate to the second substrate.

3. A microfluidic device, comprising:

a) a first and a second substrate;

b) a fluid channel;

c) at least a channel and one or more fluid ports etched in the first and/or second substrates;

d) a first layer deposited on a surface of the second substrate, from which the first layer is partially removed in accordance with a predefined geometry; and

e) a second layer deposited on top of the first layer and the surface of the second substrate;

wherein the first substrate is aligned relative to the surface of the second substrate and thereafter bonded therewith, and

wherein at least a part of the first layer is arranged relative to the channel so as to form, in operational state, a detector for detecting the transport and/or the properties of the fluid in the channel.

4. The microfluidic device according to claim 3, wherein in the channel the second layer completely covers the detector part of the first layer so as to provide a contactless detector.

5. The microfluidic device according to claim 3, wherein the detector part of the first layer is at least partly exposed so as to provide a contact detector.

6. The microfluidic device according to claim 3, wherein the second layer is planarized prior to alignment and bonding of the first substrate to the second substrate.

7. A microfluidic device, comprising:

a) a first and a second substrate;

b) a fluid channel;

c) at least a channel and one or more fluid ports etched in the first and/or second substrates;

d) a first layer deposited on a surface of the second substrate, from which the first layer is partially removed in accordance with a predefined geometry; and

e) a second layer deposited on top of the first layer and the surface of the second substrate;

wherein the first substrate is aligned relative to the surface of the second substrate and thereafter bonded therewith, and

wherein the first layer is partly exposed to the channel, the exposed parts forming electrodes for providing an electrical field to generate an electro osmotic flow of the liquid in the channel.

8. The microfluidic device according to claim 7, further comprising dielectric material arranged between said electrodes in the channel.

9. The microfluidic device according to claim 7, wherein the first and second layers are partly removed so as to form electric field electrodes exposed to the channel and so as to form a gate electrode separated from the channel by the second layer.

10. The microfluidic device according to claim 9, wherein the first substrate comprises a further gate electrode, separated from the channel by a further insulating layer.

11. The microfluidic device according to claim 7, wherein the gate electrodes are AC charged and switched synchronously.

12. The microfluidic device according to claim 7, wherein the second layer is planarized prior to alignment and bonding of the first substrate to the second substrate.

13. A microfluidic device, comprising:

a) a substrate provided with a fluid channel; and

b) a plurality of electro osmotic flow drive sections for providing electro osmotic flow in the channel, each drive section comprising electric field electrodes, exposed to the channel, and one or more gate electrodes, separated from the channel by an insulating layer, wherein the electrodes of each drive section can be controlled by control means so as to control the direction of the electro osmotic flow in the channel.

14. The microfluidic device according to claim 13, wherein the channel is formed so as to hydraulically restrict the liquid flow.

15. The microfluidic device according to claim 13, wherein at least part of the channel has a serpentine form.

16. The microfluidic device according to claim 15, wherein negatively charged gate electrodes extend on one side of the channel and positively charged gate electrodes extend on the opposite side of the channel.

17. The microfluidic device according to claim 13, wherein the gate electrodes are AC charged and switched synchronously.

18. A microfluidic device, comprising a substrate provided with a fluid channel, electric field electrodes, exposed to the channel, and one or more gate electrodes, separated from the channel by an insulating layer, for providing an electro osmotic flow of the fluid in the channel, wherein the device also comprises one or more heater elements that are positioned on or in at least one of the walls of the channel for changing the temperature of the fluid in the channel.

19. The microfluidic device according to claim 18, further comprising a functional layer, in operational state in contact with the fluid in the channel, the functional layer comprising catalytic and/or absorptive material for the purpose of enhancing a chemical reaction and/or absorbing a part of the fluid.