Microfluidic Biofuel Cell

• US 20070287034A1
• Filed: 01/19/2005
• Published: 12/13/2007
• Est. Priority Date: 03/15/2004
• Status: Active Grant

• Current Assignee: St. Louis University
• Original Assignee: Saint Louis University
• Inventors: Minteer, Shelley, Martin, Robert, Moore, Christine
• Granted Patent: US 7,709,134 B2
• Time in Patent Office: Days
• US Class Current: 429/2
• CPC Class Codes: H01M 4/8605 Porous electrodes
• CPC Class Codes: H01M 4/8857 Casting, e.g. tape casting, vacuum slip casting
• CPC Class Codes: H01M 8/1007 with both reactants being gaseous or vaporised H01M8/12 takes precedence
• CPC Class Codes: H01M 8/16 Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
• CPC Class Codes: Y02E 50/30 Fuel from waste, e.g. synthetic alcohol or diesel
• CPC Class Codes: Y02E 60/50 Fuel cells

Abstract

Microfluidic biofuel cells comprising a bioanode and/or a biocathode are formed using microfluidic principles and soft lithography. The enzymes utilized in the redox reactions at the bioanode and/or the biocathode are stabilized in a micellar or inverted micellar structure. The biofuel cell is used to produce high power densities.

Claims

1. A biofuel cell for generating electricity using a fuel fluid comprising:

a substrate;

a cathode supported by the substrate and capable of a reaction to reduce an oxidant in the presence of electrons to form water;

an anode supported by the substrate and capable of a reaction to oxidize the fuel fluid;

at least one of the anode and cathode including an enzyme for use in carrying out its respective reaction; and

either

(i) at least one of the anode and cathode being formed for flow of the fuel fluid therewithin for use in producing an electrical current;

(ii) the cathode comprising an enzyme immobilization material comprising a micellar or inverted micellar structure;

or

(iii) at least one of the anode and cathode comprising a width less than about 200 μ

m and at least one surface having an irregular, three dimensional topography capable of inducing convective flow of the fuel fluid and/or oxidant over said surface.

2. The biofuel cell of claim 1, wherein the anode comprises

(a) an electron conductor;

(b) an electron mediator, the reduced form of the electron mediator being capable of releasing electrons to the electron conductor;

(c) at least one enzyme capable of reacting with the oxidized form of the electron mediator and the fuel fluid to produce an oxidized form of the fuel fluid and a reduced form of the electron mediator;

(d) an enzyme immobilization material capable of immobilizing and stabilizing the enzyme, the material being permeable to the fuel fluid and the electron mediator, and optionally, the material comprises the electron mediator.

4. The biofuel cell of claim 1, wherein the anode comprises

(a) an electron conductor;

(b) an electron mediator;

(c) at least one enzyme capable of reacting with the oxidized form of the electron mediator and the fuel fluid to produce an oxidized form of the fuel fluid and a reduced form of the electron mediator;

(c) an enzyme immobilization material optionally comprising the electron mediator and/or an electrocatalyst, the material being capable of immobilizing and stabilizing the enzyme, the material being permeable to the fuel fluid; and

(d) an electrocatalyst adjacent the electron conductor, an oxidized form of the electrocatalyst being capable of reacting with the reduced form of the electron mediator to produce an oxidized form of the electron mediator and a reduced form of the electrocatalyst, the reduced form of the electrocatalyst being capable of releasing electrons to the electron conductor.

7. The biofuel cell claim 2 wherein the anode'"'"'s electron mediator comprises pyrroloquinoline quinone (PQQ), phenazine methosulfate, dichlorophenol, indophenol, short chain ubiquinones, or potassium ferricyanide.

11. The biofuel cell of claim 2 wherein the enzyme comprises an oxidoreductase that acts on the CH—

OH group or CH—

NH group, a dehydrogenase, alcohol dehydrogenase, aldehyde dehydrogenase, formate dehydrogenase, formaldehyde dehydrogenase, glucose dehydrogenase, glucose oxidase, lactatic dehydrogenase, lactose dehydrogenase, pyruvate dehydrogenase, or a PQQ-dependent dehydrogenase.

13. The biofuel cell of claim 1, wherein the cathode comprises

(a) an electron conductor;

(b) at least one enzyme capable of reacting with a reduced form of an electron mediator and an oxidant to produce an oxidized form of the electron mediator and water; and

(c) an enzyme immobilization material comprising either the electron mediator, an electrocatalyst, or the electron mediator and an electrocatalyst, the enzyme immobilization material being capable of immobilizing and stabilizing the enzyme, the material being permeable to the oxidant, an oxidized form of the electrocatalyst being capable of gaining electrons from the electron conductor to produce a reduced form of the electrocatalyst that is capable of reacting with an oxidized form of the electron mediator to produce a reduced form of the electron mediator and an oxidized form of the electrocatalyst.

14. The biofuel cell of claim 1, wherein the cathode comprises

(a) an electron conductor;

(b) at least one enzyme capable of reacting with a reduced form of an electron mediator and an oxidant to produce an oxidized form of the electron mediator and water; and

(c) an enzyme immobilization material optionally comprising the electron mediator, the enzyme immobilization material being capable of immobilizing and stabilizing the enzyme, the material being permeable to the oxidant, an oxidized form of the electron mediator being capable of gaining electrons from the electron conductor to produce a reduced form of the electron mediator.

34. The biofuel cell of claim 1 wherein the fuel fluid and/or the oxidant is moved through the biofuel cell at a flow rate of between about 0.01 μ

L/min and about 10 μ

L/min.

38. The biofuel cell of claim 2 wherein the enzyme immobilization material comprises a micellar or inverted micellar structure.

3. (canceled)

5-6. -6. (canceled)

8-10. -10. (canceled)

12. (canceled)

17. The biofuel cell of claim 12 wherein the enzyme comprises a laccase, an oxidase, a glucose oxidase, an alcohol-based oxidase, a cholesterol-based oxidase, an oxygen oxidoreductase, or a bilirubin oxidase.

15-16. -16. (canceled)

18-33. -33. (canceled)

35-37. -37. (canceled)

39-44. -44. (canceled)

45. An electrode comprising an electron conductor having a width less than about 200 μ

m and at least one surface having an irregular, three dimensional topography capable of inducing convective flow of the fuel fluid over said surface.

48. The electrode of claim 45, wherein the electrode has a width of between about 10 μ

m and 50 μ

m.

51. The electrode of claim 45, wherein the electrode comprises a carbon-based ink.

46-47. -47. (canceled)

49-50. -50. (canceled)

52. (canceled)

53. An electrode comprising an electron conductor having an effective surface area that is at least 1.5 times greater than its geometric surface area, wherein one dimension of the electrode is less than 100 μ

m.

54-60. -60. (canceled)

61. A method for forming an electrode for use in a biofuel cell, the method comprising

forming at least one electrical connector on a substrate;

forming at least one microchannel in a non-conductive casting mold comprised of a material that will not passivate the electrode and can be reversibly sealed to the substrate;

adhering the casting mold to the substrate;

flowing an electron conductor solution through the microchannels; and

curing the electron conductor solution to form the electrode.

62. The method of claim 61, wherein the microchannels in the casting mold are formed using soft lithography.

64. The method of claim 61, wherein the substrate is flat.

66. The method of claim 61, wherein the method further comprises removing the casting mold and replacing it with a gas-permeable mold comprising larger microchannels.

68. The method of claim 66, wherein the microchannels in the gas-permeable mold are formed using soft lithography.

70. The method of claim 61, wherein the electron conductor solution comprises a carbon-based ink.

63. (canceled)

65. (canceled)

67. (canceled)

69. (canceled)

71. (canceled)