MICRODIALYSIS PROBE

• US 20070287952A1
• Filed: 04/27/2007
• Published: 12/13/2007
• Est. Priority Date: 04/27/2006
• Status: Abandoned Application

• Current Assignee: United States Secretary of Health and Human Services is the head of the United States Department of Health and Human Services
• Original Assignee: United States Secretary of Health and Human Services is the head of the United States Department of Health and Human Services
• Inventors: Gerber, Lynn, Shah, Jay, Danoff, Jerome, Phillips, Terry
• Application Number:
  US11/741,451
• Publication Number:
  US 20070287952A1
• Time in Patent Office: Days
• US Class Current: 604/27
• CPC Class Codes: A61B 5/145 Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissuemeasuring of blood pressure or blood flow A61B5/02; non-radiation detecting or locating of foreign bodies in blood A61B5/06
• CPC Class Codes: A61B 5/14528 invasively
• CPC Class Codes: A61B 5/14539 for measuring pH
• CPC Class Codes: A61B 5/1473 invasive, e.g. introduced into the body by a catheter A61B5/1482 takes precedence
• CPC Class Codes: A61M 2005/1726 the body parameters being measured at, or proximate to, the infusion site

Abstract

Provided are novel devices, systems, and methods for performing microanalysis of a localized biochemical milieu, and/or for highly localized drug delivery and treatment evaluation.

Claims

1. A microdialysis probe comprising:

a rigid housing having a lumen and a working end;

an aperture in fluid communication with the lumen at said working end;

a plurality of tubes extending longitudinally through said lumen, each of said plurality of tubes having a terminal opening, said terminal openings being in fluid communication with the lumen near said working end; and

,

a semipermeable membrane disposed within said lumen and located between said aperture and said terminal openings.

2. The microdialysis probe of claim 1 wherein the terminal openings of the respective tubes are longitudinally offset from one another.

3. The microdialysis probe of claim 2 wherein the terminal openings are longitudinally offset by about 75 μ

m to about 125 μ

m.

4. The microdialysis probe of claim 1 wherein said semipermeable membrane is about 70 μ

m to about 250 μ

m from the nearest terminal opening of said plurality of tubes.

5. The microdialysis probe of claim 1 wherein said semipermeable membrane is about 150 μ

m to about 225 μ

m from the aperture.

6. The microdialysis probe of claim 1 wherein said semipermeable membrane comprises a 100 kDa microdialysis membrane.

7. The microdialysis probe of claim 1 wherein said rigid housing comprises a needle.

8. The microdialysis probe of claim 1 wherein said working end of said rigid housing comprises a substantially rounded tip.

9. The microdialysis probe of claim 1 further comprising a sheath that at least partially encircles said plurality of tubes at a location distal from said terminal openings.

10. The microdialysis probe of claim 1 wherein one or more of said plurality of tubes are adapted for connection to a fluid flow line.

11. A microdialysis probe comprising:

a hollow bore needle having a proximal end and a distal end, the proximal end including a substantially rounded hollow tip;

a membrane disposed within said hollow bore needle at said proximal end; and

first and second tubing at least partially disposed within said hollow bore needle and in fluid communication with said membrane.

12. The microdialysis probe of claim 11 wherein said membrane is disposed at a distance of up to 200 μ

m from said tip.

13. The microdialysis probe of claim 11 further comprising a sheath.

14. The microdialysis probe of claim 13 wherein said sheath surrounds the distal end and at least a portion of the said first and second tubing.

15. A microdialysis system comprising:

a probe, comprising

a rigid housing having a lumen and a working end;

an aperture in fluid communication with the lumen at said working end;

a plurality of tubes extending longitudinally through said lumen, each of said plurality of tubes having a terminal opening, said terminal openings being in fluid communication with the lumen near said working end; and

,

a semipermeable membrane disposed within said lumen and located between said aperture and said terminal openings;

a pump in fluid communication with at least one of said plurality of tubes; and

,

a sample collector in fluid communication with at least one other of said plurality of tubes.

16. The microdialysis system of claim 15 wherein the terminal openings of the respective tubes are longitudinally offset from one another.

17. The microdialysis system of claim 16 wherein the terminal openings are longitudinally offset by about 75 μ

m to about 125 μ

m.

18. The microdialysis system of claim 15 wherein said semipermeable membrane is about 70 μ

m to about 250 μ

m from the nearest terminal opening of said plurality of tubes.

19. The microdialysis system of claim 15 wherein said semipermeable membrane is about 150 μ

m to about 225 μ

m from the aperture.

20. The microdialysis system of claim 15 wherein said semipermeable membrane comprises a 100 kDa filter.

21. The microdialysis system of claim 15 wherein said rigid housing comprises a needle.

22. The microdialysis system of claim 15 wherein said working end comprises a substantially rounded tip.

23. The microdialysis system of claim 15 further comprising a sheath that at least partially encircles said plurality of tubes at a location distal from said terminal openings.

24. The microdialysis system of claim 15 wherein one or more of said plurality of tubes are adapted for connection to a fluid flow line.

25. A method comprising:

contacting a tissue with a microdialysis probe, said probe comprising

a rigid housing having a lumen and a working end;

an aperture in fluid communication with the lumen at said working end;

a plurality of tubes extending longitudinally through said lumen, each of said plurality of tubes having a terminal opening, said terminal openings being in fluid communication with the lumen near said working end; and

,

a semipermeable membrane disposed within said lumen and located between said aperture and said terminal openings;

a semipermeable membrane disposed transversely within said internal space, located distally from said aperture and proximally from each of the terminal openings of said plurality of lumens.

26. The method according to claim 25 wherein at least one of said plurality of tubes is in fluid communication with a pump, and at least one other of said plurality of tubes is in fluid communication with a sample collector.

27. The method according to claim 26 further comprising withdrawing a fluid from said tissue through said at least one tube in fluid communication with a sample collector.

28. The method according to claim 27 further comprising analyzing said withdrawn fluid.

29. The method according to claim 28 wherein said analyzing said withdrawn fluid comprises measuring the presence of one or more analytes.

30. The method according to claim 29 wherein said one or more analytes are selected from hydrogen ions, cytokines, growth factors, neuropeptides, kinins, vasodilators, chemokines, neurotransmittors, hormones, and cell regulatory molecules.

31. The method according to claim 26 further comprising delivering a fluid via said pump through said at least one tube in fluid communication with said pump.

32. The method according to claim 31 wherein said fluid comprises a beneficial agent.

33. The method according to claim 32 further comprising withdrawing said probe, and contacting said tissue with a second microdialysis probe, wherein at least one of said plurality of tubes of said second microdialysis probe is in fluid communication with a pump, and at least one of said plurality of tubes of said second microdialysis probe is in fluid communication with a sample collector.

34. The method according to claim 33 further comprising withdrawing a fluid from said tissue through said at least one tube in fluid communication with a sample collector.

35. The method according to claim 34 further comprising analyzing said withdrawn fluid.

36. The method according to claim 35 wherein said analyzing said withdrawn fluid comprises measuring the presence of one or more analytes.

37. The method according to claim 36 wherein said one or more analytes are selected from hydrogen ions, cytokines, growth factors, neuropeptides, kinins, vasodilators, chemokines, neurotransmittors, hormones, and cell regulatory molecules.