AGROBACTERIUM TRANSFORMATION OF GUAR

• US 20070289032A1
• Filed: 06/01/2007
• Published: 12/13/2007
• Est. Priority Date: 06/02/2006
• Status: Abandoned Application

• Current Assignee: The Samuel Roberts Noble Foundation
• Original Assignee: The Samuel Roberts Noble Foundation
• Inventors: Dixon, Richard, Vaghchhipawala, Sanchita
• Application Number:
  US11/757,164
• Publication Number:
  US 20070289032A1
• Time in Patent Office: Days
• US Class Current: 800/278
• CPC Class Codes: C12N 15/8205 Agrobacterium mediated transformation

Abstract

The invention provides methods for transforming guar plants with Agrobacterium. The invention allows creation of transgenic guar plants with improved traits.

Claims

1. A method of transforming a guar plant comprising:

(a) obtaining an explant from a guar seedling;

(b) contacting the explant with a recombinant Agrobacterium strain containing a DNA of interest to transform at least a first cell of the explant with the DNA of interest;

(c) culturing the explant to induce formation of roots;

(d) allowing roots to form from the explant; and

(e) cultivating the explant under plant growth conditions to produce a transgenic guar plant comprising the DNA of interest.

2. The method of claim 1, wherein the explant is a cotyledon explant.

3. The method of claim 1, wherein the explant comprises a cotyledonary node.

4. The method of claim 1, wherein the DNA of interest comprises a selectable marker and the explant is contacted with a selective agent following transforming the first cell with the DNA of interest.

5. The method of claim 1, wherein said guar seed is a seed of the cultivar Lewis or Kinman.

6. The method of claim 1, wherein the Agrobacterium tumefaciens strain is strain C58C1.

7. The method of claim 1, wherein culturing the explant to induce formation of roots comprises contacting the explant with Shoot Initiation Medium (SIM).

8. The method of claim 7, wherein culturing the explant to induce formation of roots comprises contacting the explant with Shoot Elongation Medium (SEM) and allowing shoots to form.

9. The method of claim 8, wherein the shoots are about 2-5 mm in length.

10. The method of claim 8, wherein the shoots are about 5-10 mm in length.

11. The method of claim 1, wherein culturing the explant to induce formation of roots comprises infection with A. rhizogenes or culturing in root induction medium.

12. The method of claim 1, wherein culturing the explant to induce formation of roots comprises culturing in Farhaeus medium for about 4 to 6 weeks.

13. The method of claim 12, wherein cultivating the explant under plant growth conditions comprises transferring the explant to soil.

14. The method of claim 11, wherein the A. rhizogenes is strain ARqua1.

15. The method of claim 1, wherein the explant is obtained from about 7 to 9 days post-germination.

16. The method of claim 1, wherein step (b) comprises co-cultivating the explant and Agrobacterium strain for about 2 days.

17. The method of claim 7, wherein the explant is contacted with the Shoot Initiation Medium for about 10 to 14 days.

18. The method of claim 17, wherein the Shoot Initiation Medium comprises a selective agent.

19. The method of claim 8, wherein the explant is contacted with the Shoot Elongation Medium (SEM) for about 7 to 14 days.

20. The method of claim 1, wherein culturing the explant to induce formation of roots comprises culturing the explant in Root Induction Medium 1 and/or Root Induction Medium 2.

21. The method of claim 1, wherein the DNA of interest confers a trait selected from the group consisting of herbicide tolerance, an insect resistance, disease resistance, pest resistance, improved nutritional quality, modified carbohydrate metabolism and modified lipid metabolism.

22. The method of claim 1, wherein the DNA of interest encodes β

-mannan synthase or α

-galactosyltransferase.

23. A guar plant generated according to the method of claim 1.

24. A progeny of the plant of claim 23.

25. A plant part of the plant of claim 23.

26. A cell of the plant of claim 23.

27. A seed of the plant of claim 23.

28. A method of producing food or feed, comprising (a) obtaining the plant of claim 23 or a part thereof;

and (b) producing food or feed from the plant or part thereof.

29. A method of breeding a plant comprising (a) obtaining the plant of claim 23;

and (b) breeding said plant with a second guar plant.