Method for Detecting and/or Identifying Bacteria of the Genus Staphylococcus
First Claim
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1. A method for detecting and/or identifying bacteria of the genus Staphylococcus in a biological sample, comprising the following steps:
- A. the nucleic acid material of the bacteria of the genus Staphylococcus is extracted, B. at least one target sequence of the nucleic acid material of the bacteria of the genus Staphylococcus is amplified using at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 1 and/or at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 2, in order to obtain amplicons of the target sequence, C. the presence of bacteria of the genus Staphylococcus is determined by detecting said amplicons.
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Abstract
The present invention relates to a method for detecting and/or identifying bacteria of the genus Staphylococcus, comprising the following steps:
- A. the nucleic acid material of the bacteria of the genus Staphylococcus is extracted,
- B. at least one target sequence of the nucleic acid material of the bacteria of the genus Staphylococcus is amplified using at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 1 and/or at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 2, in order to obtain amplicons of the target sequence,
- C. the presence of bacteria of the genus Staphylococcus is determined by detecting said amplicons.
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10 Claims
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1. A method for detecting and/or identifying bacteria of the genus Staphylococcus in a biological sample, comprising the following steps:
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A. the nucleic acid material of the bacteria of the genus Staphylococcus is extracted, B. at least one target sequence of the nucleic acid material of the bacteria of the genus Staphylococcus is amplified using at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 1 and/or at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 2, in order to obtain amplicons of the target sequence, C. the presence of bacteria of the genus Staphylococcus is determined by detecting said amplicons. - View Dependent Claims (2)
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- 3. An amplification primer, characterized in that it comprises at least 15 nucleotide motifs of SEQ ID No. 1.
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4. An amplification primer, characterized in that it comprises at least 20 nucleotide motifs of SEQ ID No. 2.
- 8. A hybridization probe, characterized in that it comprises at least 15 nucleotide motifs of SEQ ID No. 1.
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9. A hybridization probe, characterized in that it comprises at least 20 nucleotide motifs of SEQ ID No. 2.
Specification