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Nucleic Acid Detection Method Involving the Direct Generation of a Measurable Signal

  • US 20070292868A1
  • Filed: 06/16/2005
  • Published: 12/20/2007
  • Est. Priority Date: 06/16/2005
  • Status: Active Grant
First Claim
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1. Method to detect nucleic acids, DNA or RNA, comprising directly generating a measurable signal wherein the signal is produced by the activity of an enzyme with 3′

  • -5′

    nuclease activity when this makes contact with a nucleic acid substrate, with at least one oligonucleotide capable of hybridizing with it, leaving one or more bases unpaired at the 3′

    end of the chain, or adjacent bases, which are split off by the activity of the enzyme 3′

    -5′

    nuclease, generating the measurable signal.

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