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PYROPHOSPHOROLYSIS ACTIVATED POLYMERIZATION (PAP)

  • US 20070298428A1
  • Filed: 07/02/2007
  • Published: 12/27/2007
  • Est. Priority Date: 02/23/2000
  • Status: Active Grant
First Claim
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1. A method of detecting a nucleic acid which comprises:

  • (a) annealing to a nucleic acid an oligonucleotide P*, wherein the oligonucleotide P* has a non-extendable 3′

    end, wherein the 3′

    non-extendable terminus of the oligonucleotide P* is removable by pyrophosphorolysis;

    (b) removing the 3′

    non-extendable terminus of the oligonucleotide P* by pyrophosphorolysis to produce an unblocked oligonucleotide; and

    (c) detecting the removal of the 3′

    non-extendable terminus of the oligonucleotide P*.

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