Assay Device and Method
First Claim
1. A method comprising:
- accumulating an opaque material on a region of a microfluidic chamber;
exposing the region to light; and
determining the transmission of light through the opaque material.
2 Assignments
0 Petitions
Accused Products
Abstract
An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion (140) of a surface (130) of a microfluidic chamber (120,122,124); passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.
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Citations
71 Claims
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1. A method comprising:
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accumulating an opaque material on a region of a microfluidic chamber;
exposing the region to light; and
determining the transmission of light through the opaque material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. An immunoassay comprising:
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a microfluidic chamber having a surface;
at least one of an antigen or an antibody disposed on a portion of the chamber surface; and
an opaque layer associated with the portion of the chamber. - View Dependent Claims (20, 21, 22, 23, 24, 25)
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26. A method comprising:
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passing a fluid sample over a surface;
allowing a sample component to bind with a binding partner disposed on the surface;
allowing a metal colloid to associate with a sample component; and
flowing a metal solution over the surface to form a metallic layer. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
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45. A method comprising:
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flowing a fluid sample over a surface;
allowing a sample component to bind with a binding partner disposed on the surface; and
accumulating an opaque material on a portion of the surface. - View Dependent Claims (46, 47, 48, 49, 50, 51, 52, 53, 54, 55)
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56. An assay kit comprising:
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a surface including a microfluidic channel;
at least one of an antibody or an antigen associated with a portion of the microfluidic channel;
a metal colloid associated with an antibody or an antigen;
a metal precursor; and
instructions for performing the assay. - View Dependent Claims (57)
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58. A method comprising:
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contacting a sample with an antibody or an antigen;
allowing a sample component to bind with the antibody or antigen;
illuminating any bound sample component with a pulse modulated light; and
determining binding of a sample component to an antigen or antibody. - View Dependent Claims (59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71)
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Specification