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System and methods for monitoring the amplification and dissociation behavior of DNA molecules

  • US 20080003593A1
  • Filed: 11/30/2006
  • Published: 01/03/2008
  • Est. Priority Date: 06/30/2006
  • Status: Active Grant
First Claim
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1. A method, comprising:

  • (a) introducing into a microchannel at least one bolus containing nucleic acid;

    (b) forcing the bolus to move through the microchannel;

    (c) while the bolus is moving through the microchannel, amplifying nucleic acid contained in the bolus and determining whether predetermined event has occurred;

    (d) if the predetermined event has not occurred, then repeating step (c), otherwise, while the bolus is still moving through the microchannel, causing dsDNA within the bolus to transition to ssDNA and using an image sensor to capture images of the bolus.

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