APPARATUS AND METHOD FOR TESTING LIQUID SAMPLES

• US 20080003638A1
• Filed: 09/10/2007
• Published: 01/03/2008
• Est. Priority Date: 04/17/2003
• Status: Active Grant

• Current Assignee: IDEXX Laboratories Incorporated
• Original Assignee: IDEXX Laboratories Incorporated
• Inventors: Smith, Kenneth, McCarthy, Kathleen, Townsend, David, Goldman, Ross
• Granted Patent: US 7,781,185 B2
• Time in Patent Office: Days
• US Class Current: 435/40
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2300/0832 cylindrical, tube shaped
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404
• CPC Class Codes: C12Q 1/06 Quantitative determination

Abstract

An article for holding a liquefied sample for the quantification of biological material in the sample includes a device having a reaction chamber enclosing a volume therein, the reaction chamber having an upper opening through which a liquefied sample can be poured and a plurality of discrete non-permeable compartments, each of the compartments having an upper rim and being configured and dimensioned to hold separate aliquots of a liquefied sample therein; and a gasket lid removably secured to the top of the device, the gasket lid being configured and dimensioned for sealing the upper rim of each compartment to prevent liquid communication between the compartments.

Claims

1. A method for quantification of biological material in a liquefied sample, comprising the steps of:

a) providing a device having a reaction chamber enclosing a volume therein, said reaction chamber having an upper opening through which a liquefied sample can be poured and a plurality of discrete non-permeable compartments, each of said compartments having an upper rim and being configured and dimensioned to hold separate aliquots of a liquefied sample therein; and

a gasket lid removably secured to the top of said device, said gasket lid being configured and dimensioned for sealing the upper rim of each compartment to prevent liquid communication between said compartments;

b) adding a liquefied sample to said device such that the liquefied sample is distributed into said compartments;

c) securing the gasket lid on top of said device thereby sealing said compartments; and

d) detecting the presence or absence of said biological material in each of said compartment.

2. The method of claim 1, wherein the biological material to be quantified is selected from one or more bacterial species and the testing medium is a specific biological detection medium.

3. The method of claim 2, wherein said one or more bacterial species is Escherichia coli and coliform bacteria.

4. The method of claim 2, wherein the specific biological detection medium is a medium for detecting enterococci bacteria in a sample.

5. The method of claim 2, wherein the specific biological detection medium is pre-dispensed into the compartments.

6. The method of claim 2, wherein the specific biological detection medium is mixed with the liquefied sample prior to distribution into the device.

7. The method of claim 2, wherein the specific biological detection medium comprises two or more distinct media that are individually distributed in different compartments, thereby providing multiplex detection.

8. The method of claim 1, wherein the providing step further includes a diverter defining an upper surface having an edge portion disposed in direct contact with the upper rims of the plurality of compartments.

9. The method of claim 8, wherein the diverter is further disposed in fluid communication with the upper opening such that when a liquefied sample is poured through the upper opening the sample is diverted to fill each of the plurality of compartments.

10. The method of claim 1, wherein the number of compartments is between 12 and 36.

11. The method of claim 1, wherein the compartments are of substantially equal size.

12. The method of claim 1, wherein the compartments are configured and dimensioned to hold about four milliliters of liquid.

13. The method of claim 1, wherein the compartments are of trapezoidal cross-section shape.

14. The method of claim 1, wherein the compartments are arranged in a circular fashion within the device.

15. The method of claim 1, wherein the compartments comprise a first plurality of compartments of one size and a second plurality of compartments of a different size than the first size.

16. The method of claim 1, wherein the compartments comprise a third plurality of compartments of a size different from said first and second plurality of compartments.

17. The method of claim 1, wherein the liquefied sample is water.

18. The method of claim 1, wherein the device is formed at least partially from polystyrene.

19. The method of claim 1, wherein the gasket lid has both internal and external bayonet style closures.