Directed Evolution and Selection Using in Vitro Compartmentalization

• US 20080004436A1
• Filed: 11/15/2005
• Published: 01/03/2008
• Est. Priority Date: 11/15/2004
• Status: Abandoned Application

• Current Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem), Yeda Research and Development Co. Ltd. (Weizmann Institute of Science)
• Original Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem), Yeda Research and Development Co. Ltd. (Weizmann Institute of Science)
• Inventors: Bernath, Kalia, Peisajovich, Sergio, Magdassi, Shlomo, Tawfik, Dan
• Application Number:
  US11/718,929
• Publication Number:
  US 20080004436A1
• Time in Patent Office: Days
• US Class Current: 536/23.1
• CPC Class Codes: C12N 15/1075 by coupling phenotype to genotype, not provided for in other groups of this subclass

Abstract

The present invention is related to the field of compartmentalized libraries of genetic elements and the selection of biologically active molecules and the genes encoding same from said libraries. The selection assay of the invention utilizes water-in-oil emulsions and is particularly advantageous in applications in the field of directed-evolution, as exemplified herein for selection of protein inhibitors of DNA nucleases.

Claims

1. A library of genetic elements encoding gene products, the library being compartmentalized in aqueous droplets of a water-in-oil emulsion, wherein each aqueous droplet comprises the components necessary to express gene products encoded by the genetic elements and further comprises at least one biological moiety the activity of which results in the modification of said genetic elements or the gene products encoded by said genetic elements.

2. The library of claim 1, wherein the at least one biologically active moiety is not activated.

3. The library of claim 1, wherein each aqueous droplet further comprises at least one activating agent capable of activating the biologically active moiety.

4. The library of claim 3, wherein the at least one biologically active moiety is selected from the group consisting of:

a protein, a polypeptide and a peptide.

5. The library of claim 4, wherein the at least one biologically active moiety is an enzyme.

6. The library of claim 5, wherein the at least one biologically active moiety is a nuclease.

7. The library of claim 5, wherein the at least one activating agent is selected from the group consisting of:

inorganic or organic salts, monosaccharides, disaccharides, oligosaccharides, amino acids, peptides, polypeptides, nucleotides, nucleosides, oligonucleotides, polynucleotides, vitamins and small organic molecules.

8. The library of claim 6, wherein the at least one activating agent is a bivalent salt.

9. A method for selecting genetic elements encoding gene products of a desired activity, the method comprising:

a) providing a library of genetic elements;

b) providing at least one biologically active moiety the activity of which results in the modification of said genetic elements or the gene products encoded by said genetic elements;

c) co-compartmentalizing the genetic elements with the at least one biologically active moiety into aqueous droplets, the aqueous droplets being the internal discontinuous phase of a water-in-oil emulsion, such that each aqueous droplet comprises at least one genetic element together with the at least one biologically active moiety and further comprises components necessary to express the gene products encoded by said at least one genetic element;

d) merging the water-in-oil emulsion with micelles comprising at least one activating agent capable of modulating the activity of said at least one biological moiety; and

e) detecting genetic elements encoding gene products having a desired activity.

10. The method of claim 9, further comprising prior to merging the water-in-oil emulsion with the micelles, the step of:

incubating the water-in-oil emulsion under conditions enabling expression of said gene products.

11. The method of claim 9, further comprising following merging the water-in-oil emulsion with the micelles, the steps of:

coalescing the water-in-oil emulsion thereby forming a continuous aqueous phase from the droplets; and

detecting in the aqueous phase genetic elements which encode the desired gene products.

12. The method of claim 9, wherein detecting the genetic elements comprises amplifying said genetic elements using PCR techniques and detecting the amplified products.

13. The method of claim 11, wherein the aqueous phase is re-emulsified prior to amplification.

14. The method of claim 11, wherein the aqueous phase is re-emulsified in oil comprising a surfactant capable of maintaining the integrity of the water-in-oil emulsion at temperatures within the range of 65°

C. to 100°

C.

15. The method of claim 14, wherein the surfactant is a polymer having a Hydrophilic-Lipophilic Balance value below 10.

16. The method of claim 14, wherein the surfactant is high molecular weight modified polyether polysiloxane.

17. The method of claim 14, wherein the surfactant is selected from the group consisting of:

cetyl dimethicone copolyol, polysiloxane polyalkyl polyether copolymer, cetyl dimethicone copolyol, polyglycerol ester, poloxamer and polyvinyl pyrrolidone/hexadecane copolymer.

18. The method of claim 14, wherein the surfactant is cetyl dimethicone copolyol.

19. The method of claim 13, wherein the ratio of said surfactant to the oil is within the ranges of 1-20% v/v.

20. The method of claim 9, wherein the micelles comprise from 100 to 400 volumes of oil, and from 10 to 40 volumes of total surfactant to every one volume of an aqueous phase containing the at least one activating agent.

21. The method of claim 20, wherein the micelles have a mean droplet size in the range of 0.01 micron to 1 micron.

22. The method of claim 21, wherein the mean droplet size is approximately 0.1 micron.

23. The method of claim 9, wherein the at least one biologically active moiety is selected from the group consisting of:

an enzyme, a protein, a polypeptide and a peptide.

24. The method of claim 23, wherein the biologically active moiety is a nuclease.

25. The method of claim 9, wherein the at least one activating agent is selected from the group consisting of:

inorganic or organic salts, monosaccharides, disaccharides, oligosaccharides, amino acids, peptides, polypeptides, nucleotides, nucleosides, oligonucleotides, polynucleotides, vitamins and small organic molecules.

26. The method of claim 24, wherein the at least one activating agent is a bivalent salt.

27. A gene product selected according to the method of claim 9.

28. The gene product of claim 27, being a nuclease inhibitor.