SCAFFOLD-FREE SELF-ORGANIZED 3D SYNTHETIC TISSUE
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Accused Products
Abstract
The present invention can be used for actual implantation surgery without a scaffold. The present invention provides a synthetic tissue or complex which can be produced by culture and has a high level of differentiation ability. The present invention also provides a therapy and medicament for repairing and/or regenerating tissue using replacement and covering. By culturing cells under specific culture conditions such that medium contains an extracellular matrix synthesis promoting agent, the cells are organized and are easily detached from a culture dish. The present invention was achieved by finding such a phenomenon. In addition, the self contraction of the tissue can be regulated by culturing the tissue in a suspended manner. Therefore, it is possible to regulate the three-dimensional shape of the tissue. The present invention also provides a method for producing an implantable synthetic tissue which does not require a plurality of monolayer cell sheets assembled to form a three-dimensionally structured synthetic tissue. The present invention is characterized by richness in adhesion molecules, nonnecessity of additional fixation at an implantation site, and good biological integration.
27 Citations
36 Claims
- 1. An implantable synthetic tissue.
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29. A method for producing a synthetic tissue, comprising the steps of:
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A) providing cells;
B) placing the cells in a container, the container having cell culture medium containing an ECM synthesis promoting agent and having a sufficient base area which can accommodate a synthetic tissue having a desired size;
C) culturing the cells in the container along with the cell culture medium containing the ECM synthesis promoting agent for a period of time sufficient for formation of the synthetic tissue having the desired size; and
D) detaching the cells from the container.
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30. A cell culture composition for producing a synthetic tissue from cells, comprising:
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A) an element for maintaining the cells; and
B) an extracellular matrix synthesis promoting agent.
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31. A complex for reinforcing a portion of an organism, comprising cells and a component derived from the cells.
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32. A method for reinforcing a portion of an organism, comprising the steps of:
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A) replacing the portion with a complex comprising cells and a component derived from the cells or providing the complex to cover the portion, or both; and
B) holding the complex for a sufficient period of time for biologically adhering the complex to the portion.
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33. A method for treating a portion of an organism, comprising the steps of:
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A) replacing the portion with a complex comprising cells and a component derived from the cells or providing the complex to cover the portion, or both; and
B) holding the complex for a sufficient period of time for restoring a condition of the portion.
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34. A method for producing a synthetic tissue, comprising the steps of:
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A) providing cells;
B) placing the cells in a container, the container having cell culture medium containing an ECM synthesis promoting agent and having a sufficient base area which can accommodate a synthetic tissue having a desired size;
C) culturing the cells in the container along with the cell culture medium containing the ECM synthesis promoting agent for a period of time sufficient for formation of the synthetic tissue having the desired size; and
D) regulating a thickness of the synthetic tissue by a physical or chemical stimulus to a desired thickness.
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35. A tissue complex, comprising an implantable synthetic tissue and another synthetic tissue.
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36. A composition for use in producing a synthetic tissue having a desired thickness, comprising a chemical substance selected from the group consisting of actin depolymerizing agents and actin polymerizing agents.
Specification