TUMOR-TARGETED OPTICAL CONTRAST AGENTS
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Accused Products
Abstract
Cyanine dye bioconjugates useful for diagnostic imaging and therapy are disclosed. The conjugates include several cyanine dyes with a variety of bis- and tetrakis (carboxylic acid) homologs. The compounds may be conjugated to bioactive peptides, carbohydrates, hormones, drugs, or other bioactive agents. The small size of the compounds allows more favorable delivery to tumor cells as compared to larger molecular weight imaging agents. The various dyes are useful over the range of 350 to 1300 nm, the exact range being dependent upon the particular dye. The use of dimethylsulfoxide helps to maintain the fluorescence of the compounds. The inventive compounds are useful for diagnostic imaging and therapy, in endoscopic applications for the detection of tumors and other abnormalities, for localized therapy, for photoacoustic tumor imaging, detection and therapy, and for sonofluoresence tumor imaging, detection and therapy.
15 Citations
20 Claims
- 1. A compound of formula
- 6. A composition comprising a compound of formula
-
12. A method for performing a diagnostic or therapeutic procedure comprising
administering to an individual an effective amount of the compound of formula wherein W4 and X4 are independently selected from the group consisting of — - O—
, —
NR3, and —
Se—
;
Y4 is selected from the group consisting of —
(CH2)a—
CONH-Bm, —
CH2—
(CH2OCH2)b—
CH2—
CONH-Bm, —
(CH2)a—
NHCO-Bm, —
CH2—
(CH2OCH2)b—
CH2—
NHCO-Bm, —
(CH2)a—
N(R3)—
(CH2)b—
CONH-Bm, (CH2)a—
N(R3)—
(CH2)c—
NHCO-Bm, —
(CH2)a—
N(R3)—
CH2—
(CH2OCH2)b—
CH2—
CONH-Bm, —
(CH2)a—
N(R3)—
CH2—
(CH2OCH2)b—
CH2—
NHCO-Bm, —
CH2—
(CH2OCH2)b—
CH2—
N(R3)—
(CH2)a—
CONH-Bm, —
CH2—
(CH2OCH2)b—
CH2—
N(R3)—
(CH2)a—
NHCO-Bm, —
CH2—
(CH2OCH2)b—
CH2—
N(R3)—
CH2—
(CH2OCH2)d—
CONH-Bm, —
CH2—
(CH2OCH2)b—
CH2—
N(R3)—
CH2—
(CH2OCH2)d—
NHCO-Bm, —
(CH2)a—
NR3R4, and —
CH2(CH2OCH2)b—
CH2NR3R4;
Z4 is selected from the group consisting of —
(CH2)a—
CONH-Dm, —
CH2—
(CH2OCH2)b—
CH2—
CONH-Dm, —
(CH2)a—
NHCO-Dm, —
CH2—
(CH2OCH2)b—
CH2—
NHCO-Dm, —
(CH2)a—
N(R3)—
(CH2)b—
CONH-Dm, —
(CH2)a—
N(R3)—
(CH2)c—
NHCO-Dm, —
(CH2)a—
N(R3)—
CH2—
(CH2OCH2)b—
CH2—
CONH-Dm, —
(CH2)a—
N(R3)—
CH2—
(CH2OCH2)b—
CH2—
NHCO-Dm, —
CH2—
(CH2OCH2)b—
CH2—
N(R3)—
(CH2)a—
CONH-Dm, —
CH2—
(CH2OCH2)b—
CH2—
N(R3)—
(CH2)a—
NHCO-Dm, —
CH2—
(CH2OCH2)b—
CH2—
N(R3)—
CH2—
(CH2OCH2)d—
CONH-Dm, —
CH2—
(CH2OCH2)b—
CH2—
N(R3)—
CH2—
(CH2OCH2)d—
NHCO-Dm, —
(CH2)a—
NR3R4, and —
CH2(CH2OCH2)b—
CH2NR3R4;
A2 is a single or a double bond;
B2, C2, and D2 are independently selected from the group consisting of —
O—
, —
S—
, —
Se—
, —
P—
, —
CR1R2, —
CR1, alkyl, NR3, and —
C═
O;
A2, B2, C2, and D2 may together form a 6- to 12-membered carbocyclic ring or a 6- to 12-membered heterocyclic ring optionally containing one or more oxygen, nitrogen, or sulfur atom;
a4 and b4 are independently from 0 to 5;
R1 to R4, and R45 to R57 are independently selected from the group consisting of hydrogen, C1-C10 alkyl, C5-C20 aryl, C1-C10 alkoxyl, C1-C10 polyalkoxyalkyl, C1-C20 polyhydroxyalkyl, C5-C20 polyhydroxyaryl, C1-C10 aminoalkyl, glucose derivatives of R groups, cyano, nitro, halogen, saccharide, peptide, —
CH2(CH2OCH2)b—
CH2—
OH, —
(CH2)a—
CO2H, —
(CH2)a—
CONH-Bm, —
CH2—
(CH2OCH2)b—
CH2—
CONH-Bm, —
(CH2)a—
NHCO-Bm, —
CH2—
(CH2OCH2)b—
CH2—
NHCO-Bm, —
(CH2)a—
OH and —
CH2—
(CH2OCH2)b—
CO2H;
Bm and Dm are independently selected from the group consisting of a bioactive peptide, a protein, a cell, an antibody, an antibody fragment, a saccharide, a glycopeptide, a peptidomimetic, a drug, a drug mimic, a hormone, a metal chelating agent, a radioactive or nonradioactive metal complex, and an echogenic agent;
a and c are independently from 1 to 20; and
b and d are independently from 1 to 100, and performing the diagnostic or therapeutic procedure - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20)
- O—
Specification