METHOD AND DEVICE FOR IDENTIFYING A POLYMER SEQUENCE

• US 20080009013A1
• Filed: 09/21/2007
• Published: 01/10/2008
• Est. Priority Date: 07/19/2000
• Status: Abandoned Application

• Current Assignee: Georg Bauer, Harald Walter, Jorg Hassmann, Thomas Schalkhammer, Wolf Bertling
• Original Assignee: Georg Bauer, Harald Walter, Jorg Hassmann, Thomas Schalkhammer, Wolf Bertling
• Inventors: Walter, Harald, Bauer, Georg, Hassmann, Jorg, Bertling, Wolf, Schalkhammer, Thomas
• Application Number:
  US11/859,349
• Publication Number:
  US 20080009013A1
• Time in Patent Office: Days
• US Class Current: 435/006
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2565/515 characterised by the interaction between or sequential use of two or more arrays
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

The invention relates to a method for identifying a first polymer sequence bound to a first phase that reflects electromagnetic waves. The inventive method includes the following steps: a) bringing the first polymer sequence into contact with an affine second polymer sequence, which is directly or indirectly bound, via metallic clusters, to a solid second phase that is permeable to electromagnetic waves; b) radiating electromagnetic waves through the second phase, and; c) detecting the alteration of the properties of the reflected electromagnetic waves.

Claims

1. A device for identifying a first polymer sequence, wherein said first polymer sequence is bound to a label made of a metal foil which can be firmly linked to an object to be labeled, wherein said label made of a metal foil reflects electromagnetic waves,

said device comprising a second polymer sequence bound to a phase via metallic clusters, wherein said phase is permeable to electromagnetic waves, wherein said device can be brought into contact with a labeled object,

wherein when said first polymers and said second polymers are brought into contact with one another and are complementary to one another, a distance is established between the metal foil and the metallic clusters that results in an observable change in the optical property of the electromagnetic waves, wherein said change in optical property is a spectral shift, wherein when said first polymers and said second polymers are not complementary to one another, a distance is not established between the metal foil and the metallic clusters and does not result in the spectral shift.

2. The kit of claim 1, wherein said metallic clusters are formed from silver, gold, platinum, aluminum, copper, zinc, or indium.

3. The kit of claim 1, wherein said electromagnetic waves are light.

4. The kit of claim 3, wherein said light is generated by a fluorescent lamp, a xenon lamp, a fluorescent tube, a light emitting diode, or a laser.

5. The kit of claim 1, wherein said label made of a metal foil and said phase possess a smooth surface.

6. The kit of claim 3, further comprising means for determining the optical property of the reflected light.

7. The kit of claim 6, wherein said means for determining the optical property of the reflected light can be used for measuring the absorption in a predetermined spectral range before and/or after said first polymer sequence and said second polymer sequence have been brought into contact.

8. The kit of claim 6, wherein said means for determining the optical property of the reflected light can be used to measure the spectral shift of said reflected light.

9. The kit of claim 6, wherein said means for determining the optical property of the reflected light can be used to measure said optical property under several angles of incidence which differ from each other.

10. The kit of claim 1, wherein said first polymer sequence and/or said second polymer sequence is selected from the group consisting of DNA, RNA, proteins, peptides, peptide nucleic acids (PNA), a structurally related oligomer or polymer or a ligand thereof, wherein said oligomer or polymer is formed from one monomer or from different monomers coupled in a defined sequence.

11. The kit of claim 1, wherein the first polymer sequence and/or the second polymer sequence is/are single-stranded DNA, single-stranded RNA, or synthetic analogs thereof.