×

Protein-protein interactions and methods for identifying interacting proteins and the amino acid sequence at the site of interaction

  • US 20080009068A1
  • Filed: 02/09/2007
  • Published: 01/10/2008
  • Est. Priority Date: 05/14/1999
  • Status: Abandoned Application
First Claim
Patent Images

1. A method of identifying a compound that modulates the binding of a polypeptide to a peptide in a chosen protein, the peptide region being adjacent to a repulsive peptide region of the chosen protein, the method comprising:

  • (a) providing a set of short overlapping peptides spanning a complete sequence of at least a domain of the chosen protein, the set of short overlapping peptides being covalently attached to a support;

    (b) contacting the support to which the overlapping peptides are covalently attached with a candidate compound and the polypeptide under conditions enabling binding between the peptide attached to the support and the polypeptide;

    (c) washing the support to remove unbound polypeptides of the mixture; and

    (d) detecting binding of the polypeptide to the peptide attached to the support, wherein a change in the binding of the polypeptide to the peptide attached to the support in the presence of the candidate compound compared to the binding of the polypeptide to the peptide attached to the support in the absence of the candidate compound identifies the candidate compound as a compound that modulates binding of the polypeptide to the peptide in the chosen protein.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×