Indicator for Multiwell Plate and Method for Using the Same

• US 20080009074A1
• Filed: 05/13/2004
• Published: 01/10/2008
• Est. Priority Date: 05/13/2003
• Status: Abandoned Application

• Current Assignee: Amos Valinsky
• Original Assignee: Amos Valinsky
• Inventors: Valinsky, Amos
• Application Number:
  US10/556,741
• Publication Number:
  US 20080009074A1
• Time in Patent Office: Days
• US Class Current: 436/180
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B01L 9/56 Means for indicating position of a recipient or sample in an array
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The present invention presents a novel indicator for a multiwell plate (10) having a predetermined array of wells, and a multiwell comprising the same. The indicator comprising a pierceable foil member (20) adapted to cover the top portion of the plate, and an array of signs located on the upper surface of said foil member, adapted to indicate the location of the said array of the wells. The foil is adapted to cover said plate so at least a portion of said array of signs is overlapping said array of wells, so when the foil member is pierced by a means of a pipette (42) at the location of said sign, a noticeable deformation is obtained at the upper surface of said foil member and a clear indication is provided. The present invention is also providing for a method for indicting each sampled well in aforesaid multiwell plate.

Claims

1. An indicator for a multiwell plate consisting of a predetermined array of wells, said indicator comprising

a. a pierceable foil member adapted to cover the top portion of the plate;

b. an array of signs located on the upper surface of said foil member, adapted to indicate the location of the said array of the wells;

wherein said foil is adapted to cover said plate so at least a portion of said array of signs overlaps said array of wells, so when the foil member is pierced by a means of a pipette at the location of said sign, a noticeable indentation is obtained at the upper surface of said foil member and a clear indication is provided.

2. The indicator according to claim 1, wherein the pierceable foil member is surrounded by a frame adapted to fit in size and shape the perimeter of the multiwell plate.

3. The indicator according to claim 2, wherein the frame is made of metal substances, polymeric compositions or a combination thereof.

4. The indicator according to claim 2, wherein the frame is either elastic or rigid.

5. The indicator according to claim 1, wherein the pierceable foil member is made of metallic substances, polymeric compositions or a combination thereof.

6. The indicator according to claim 5, wherein the pierceable foil member is made of aluminum foil.

7. The indicator according to claim 1, wherein the pierceable foil member is a perforated foil.

8. The indicator according to claim 7, wherein the pierceable foil member is a perforated foil, comprising an array of punctures;

said may is adapted to overlap at least a portion of the array of the wells.

9. The indicator according to claim 8, wherein the diameter of the punctures of the perforated foil is smaller than the diameter of the well'"'"'s internal bore.

10. The indicator according to claim 1, wherein the pierceable foil member comprises at least one tiring line adjacent to the signs, so at the time said foil is pierced, it is tired at a predetermined measure, size or shape along said one or more tiring lines.

11. The indicator according to claim 1 as described in FIG. 2.

12. The indicator according to claim 2 as described in FIG. 4.

13. The indicator according to claim 1, comprising one or more of the signs described in FIG. 6.

14. A multiwell plate comprising the indicator as defined in claim 1.

15. A method for indicating each sampled well in a multiwell plate comprising;

a. mounting the pierceable foil member on top of the multiwell plate in such a manner that the array of the signs overlaps at least a portion of the array of the wells;

b. piercing said foil by a pipette, so the foil is indented in a noticeable manner; and

c. sampling the well by a means of delivering the sample from the pipette to the inner portion of the well or vice versa;

characterized in that by said indentation of the foil, each sampled well is effectively indicated and faulty actions of either double sampling or abstained sampling are avoided.