BIOSENSOR ELEMENT AND METHOD FOR MANUFACTURING THE SAME
First Claim
Patent Images
1. A biosensor element comprising:
- a metal particle immobilized on a surface of a carrier;
a probe molecule immobilized on a surface of the metal particle; and
a fluorescent molecule modified on the probe molecule.
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Accused Products
Abstract
A biosensor is formed by immobilizing metal particles immobilized on a surface of a carrier and immobilizing probe molecules which are modified with fluorescent molecules on the metal particles. A biomolecule is detected at high sensitivity by use of this biosensor and utilizing fluorescence-quenching and fluorescence-enhancement effects attributable to the metal particle. In this way, it is possible to omit amplification of the biomolecule in a specimen and fluorescence-labeling on the biomolecule when detecting the biomolecule with the biosensor. It is also possible to improve quantitative reliability and repeatability of the biosensor.
18 Citations
29 Claims
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1. A biosensor element comprising:
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a metal particle immobilized on a surface of a carrier; a probe molecule immobilized on a surface of the metal particle; and a fluorescent molecule modified on the probe molecule. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A biosensor element comprising:
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a metal particle immobilized on a surface of a carrier; and a probe molecule immobilized on a surface of the metal particle, the probe molecule being configured to bind to a fluorescence-labeled target molecule, wherein a diameter of the metal particle is in a range from 10 nm to 500 nm inclusive. - View Dependent Claims (16)
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17. A method of manufacturing a biosensor element having a probe molecule immobilized on a surface of a carrier, the method comprising the steps of:
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immobilizing a metal particle on the surface of the carrier; immobilizing a blocking agent molecule on the surface of the carrier; and immobilizing the probe molecule modified with a fluorescent molecule on a surface of the metal particle. - View Dependent Claims (18, 19, 20, 21, 22)
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23. A method of detecting a biomolecule by use of a biosensor element having a metal particle immobilized on a surface of a carrier, a probe molecule immobilized on a surface of the metal particle and a fluorescent molecule modified on the probe molecule, wherein the method comprising the steps of:
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bringing the probe molecule of the biosensor element and an unlabeled detection target biomolecule into a reaction; irradiating excitation light on the biosensor element after the reaction; and detecting fluorescence emitted from a region where the probe molecule is immobilized. - View Dependent Claims (24, 25)
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26. A method of detecting a biomolecule by use of a biosensor element having a metal particle immobilized on a surface of a carrier, a probe molecule immobilized on a surface of the metal particle and a fluorescent molecule modified on the probe molecule, the method comprising the steps of:
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irradiating excitation light on the biosensor element; measuring first intensity of fluorescence emitted from a region of the biosensor element on which the probe molecule is immobilized; bringing the probe molecule of the biosensor element and an unlabeled detection target biomolecule to a reaction; irradiating the excitation light on the biosensor element after the reaction; measuring second intensity of fluorescence emitted from the region of the biosensor element on which the probe molecule is immobilized; and calculating a contrast by dividing the second intensity of fluorescence by the first intensity of fluorescence. - View Dependent Claims (27)
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28. A method of detecting a biomolecule by use of a biosensor element having a metal particle, with a diameter in a range from 10 nm to 500 nm, immobilized inclusive on the surface of a carrier, and a probe molecule immobilized on a surface of the metal particle, the method comprising the steps of:
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bringing the probe molecule of the biosensor element and a fluorescence-labeled biomolecule to a reaction; irradiating excitation light on the biosensor element; and measuring intensity of fluorescence emitted from a region of the biosensor element on which the probe molecule is immobilized. - View Dependent Claims (29)
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Specification