METHOD FOR STORING AND REACTING IONS IN A MASS SPECTROMETER

• US 20080014656A1
• Filed: 06/27/2007
• Published: 01/17/2008
• Est. Priority Date: 06/30/2006
• Status: Active Grant

• Current Assignee: DH Technologies Development Pte. Ltd. (Danaher Corporation)
• Original Assignee: MDS Incorporated (Molecular Devices), Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Thomson, Bruce
• Granted Patent: US 7,759,637 B2
• Time in Patent Office: Days
• US Class Current: 436/173
• CPC Class Codes: H01J 49/0072 by ion/ion reaction, e.g. electron transfer dissociation, proton transfer dissociation
• CPC Class Codes: H01J 49/063 Multipole ion guides, e.g. quadrupoles, hexapoles
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

A method of analyzing ions is provided having a first ion guide with first and second ends and introducing a first group of ions and a second group of ions of opposite polarity into the first ion guide, and applying an RF voltage potential to the first ion guide for confining the first and second groups of ions radially within the first ion guide. A first trapping barrier is provided to the first end of the first ion guide for trapping the first group of ions within the first ion guide and a second trapping barrier is provided to the second end of the first ion guide for trapping the second group of ions within the first ion guide and an axial field is provided for pushing the first group of ions toward the first trapping barrier and pushing the second group of ions toward the second trapping barrier.

Claims

1. A method of analyzing ions with a mass spectrometer, the method comprising:

a. providing a first ion guide having a first end and a second end;

b. introducing a first group of ions and a second group of ions into the first ion guide, the second group of ions being opposite in polarity to the first group of ions;

c. applying an RF voltage potential to the first ion guide for confining the first group of ions and the second group of ions radially within the first ion guide;

d. providing a trapping barrier to the second end of the first ion guide for trapping the first group of ions within the first ion guide;

e. providing an axial field for pushing the first group of ions toward the trapping barrier.

2. The method of claim 1 wherein the mass spectrometer comprises an ion optical element adjacent to the second end on the first ion guide, and step (d) comprises providing the trapping barrier by applying a DC voltage potential to the ion optical element to repel the first group of ions away from the second end of the first ion guide.

3. The method of claim 1 wherein the mass spectrometer comprises an ion optical element adjacent to the second end of the first ion guide and step (d) comprises providing the trapping barrier by applying an AC voltage potential between the second end of the first ion guide and the ion optical element.

4. The method of claim 1 wherein the mass spectrometer comprises an ion optical element adjacent to the second end of the first ion guide and step (d) comprises providing the trapping barrier by applying an AC voltage potential to the ion optical element.

5. The method of claim 2 comprising configuring the DC voltage potential for attracting the second group of ions toward the second end of the first ion guide and allowing the second group of ions to continuously flow and mix with the first group of ions and ejecting the second group of ions from the first ion guide.

6. The method of claim 5 further comprising after ejecting the second group of ions from the first ion guide, configuring the DC voltage potential for attracting the first group of ions toward the second end of the first ion guide and ejecting the first group of ions from the first ion guide.

7. The method of claim 2 wherein the ion optical element comprises an aperture lens.

8. The method of claim 2 wherein the ion optical element comprises a second ion guide.

9. The method of claim 1 wherein the first ion guide comprises multipole rods.

10. The method of claim 9 wherein the multipole is selected from the group comprising of quadrupole, hexapole, and octapole rods.

11. The method of claim 1 wherein the axial field is provided by tilted rods.

12. The method of claim 1 wherein the axial field is provided by auxiliary electrodes.

13. The method of claim 1 wherein the axial field is provided by applying different potentials on segmented multipoles.

14. The method of claim 1 wherein the axial field is provided by applying different potentials on RF ring guide plates.

15. The method of claim 1 wherein the first and second group of ions are introduced sequentially.

16. The method of claim 1 wherein the first and second group of ions are introduced simultaneously.

17. The method of claim 1 wherein the first ion guide is operated at a gas pressure of between about 10 Torr and about 1×

10<sup>−

5</sup> Torr.

18. The method of claim 1 wherein the first ion guide is operated at a gas pressure of between about 1 Torr and about 1×

10<sup>−

3</sup> Torr.

19. A method of analyzing ions with a mass spectrometer, the method comprising:

a. providing a first ion guide having a first end and a second end;

b. introducing a first group of ions and a second group of ions into the first ion guide, the second group of ions being opposite in polarity to the first group of ions;

c. applying an RF voltage potential to the first ion guide for confining the first group of ions and the second group of ions radially within the first ion guide;

d. providing a first trapping barrier to the first end of the first ion guide for trapping the first group of ions within the first ion guide; and

e. providing a second trapping barrier to the second end of the first ion guide for trapping the second group of ions within the first ion guide; and

f. providing an axial field for pushing the first group of ions toward the first trapping barrier and pushing the second group of ions toward the second trapping barrier.

20. The method of claim 19 wherein the mass spectrometer comprises a first ion optical element adjacent to the first end of the first ion guide and wherein the mass spectrometer comprises a second ion optical element at the second end of the first ion guide and step (d) comprises providing the first trapping barrier by applying a first DC voltage potential to the first ion optical element and step (e) comprises providing the second trapping barrier by applying a second DC voltage potential to the second ion optical element.

21. The method of claim 20 further comprising reversing the first and second DC voltage potentials and the direction of the axial field to push the first group of ions toward the second trapping barrier and to push the second group of ions toward the first trapping barrier such that the first group of ions and the second group of ions mix and move to opposite ends of the first ion guide.

22. The method of claim 21 wherein the first and second DC voltage potentials and the axial field potential are further reversed one or more times to facilitate further interaction of the first and second groups of ions.

23. The method of claim 19 wherein the mass spectrometer comprises a first ion optical element adjacent to the first end of the first ion guide and wherein the mass spectrometer comprises a second ion optical element adjacent to the second end of the first ion guide;

step (d) comprises providing the first trapping barrier by applying a first AC voltage potential between the first end of the first ion guide and the first ion optical element; and

step (e) comprises providing the second trapping barrier by applying a second AC voltage potential between the second end of the first ion guide and the second ion optical element.

24. The method of claim 19 wherein the mass spectrometer comprises a first ion optical element adjacent to the first end of the first ion guide and wherein the mass spectrometer comprises a second ion optical element adjacent to the second end of the first ion guide and step (d) comprises applying an AC voltage potential to one or both of the ion optical elements at the first and second ends of the first ion guide.

25. The method of claim 23 or 24 wherein the axial field is turned off to allow the first and second group of ions to further mix and interact within the ion guide 26. The method of claim 23 or 24 wherein the axial field is reversed one or more times in order to cause trapped ions at each end to move toward the opposite end and mix and interact.

27. The method of claims 21, 22, 25 or 26 wherein after the first and the second group of ions have mixed and interacted, the second trapping barrier potential is reduced in order to eject the second group of ions from the first ion guide for mass analysis.

28. The method of claim 27 further comprising configuring the axial field for attracting the first group of ions towards the second end of the first ion guide and ejecting the first group of ions from the first ion guide for mass analysis after the second group of ions is ejected from the first ion guide.

29. The method of claim 20 wherein the first and second ion optical elements comprise an aperture lens.

30. The method of claim 20 wherein the first and second ion optical elements comprise a second ion guide.

31. The method of claim 19 wherein the first ion guide comprises multipole rods.

32. The method of claim 31 wherein the multipole is selected from the group comprising of quadrupole, hexapole, and octapole rods.

33. The method of claim 19 wherein the axial field is provided by tilted rods.

34. The method of claim 19 wherein the axial field is provided by auxiliary electrodes.

35. The method of claim 19 wherein the axial field is provided by applying different potentials on segmented multipoles.

36. The method of claim 19 wherein the axial field is provided by applying different potentials on RF ring guide plates.

37. The method of claim 19 wherein the first and second group of ions are introduced sequentially.

38. The method of claim 19 wherein the first and second group of ions are introduced simultaneously.

39. The method of claim 19 wherein the first ion guide is operated at a gas pressure ranging of between about 10 Torr and about 1×

10<sup>−

5</sup> Torr.

40. The method of claim 19 wherein the first ion guide is operated at a gas pressure of between about 1 Torr and about 1×

10<sup>−

3</sup> Torr.

41. A method of analyzing ions with a mass spectrometer, the method comprising:

a. providing a first ion guide having a first end and a second end;

b. introducing a first group of ions and a second group of ions into the first ion guide, the second group of ions being opposite in polarity to the first group of ions;

c. applying an RF voltage potential to the first ion guide for confining the first group of ions and the second group of ions radially within the first ion guide; and

d. providing more than one trapping regions within the first ion guide for trapping the first and second groups of ions to be trapped in separate regions of the ion guide.

42. The method of claim 41 wherein step (d) comprises providing the more than one trapping regions by providing multiple axial fields within the ion guide directed along the axis where the direction of the axial fields is reversed at one or more points within the ion guide.

43. The method of claim 42 further providing a first ion optical element at the first end of the ion guide and a second ion optical element at the second end of the ion guide.

44. The method of claim 43 wherein step (d) further comprises applying DC voltages to the first and second ion optical elements at the first and second ends of the ion guide.

45. The method of claim 43 wherein step (d) further comprises applying AC voltages to the first and second ion optical elements at the first and second ends of the ion guide

46. The method of claim 44 wherein after trapping the first and second group of ions of opposite polarities in different regions of the ion guide, the axial field and DC voltages are reversed in order to cause the first group and the second group of ions of opposite polarities to move towards each other.

47. The method of claim 45 wherein after trapping the first and second group of ions of opposite polarities in different regions of the ion guide, the axial field is reversed in order to cause the first group and the second group of ions of opposite polarities to move towards each other.

48. The method of claims 46 or 47 wherein the first and second group of ions of opposite polarities pass through the same region of the ion guide so as to interact and react with one another.

49. A method of trapping ions of opposite polarity within an ion guide comprising providing an axial field within the ion guide and alternating the direction of the axial field in time with a period that is less than the drift time of the ions that are desired to be trapped from one end of the ion guide to the other end so that most of the ions remain trapped.