Integrated Systems and Methods for Diversity Generation and Screening
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Accused Products
Abstract
Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.
109 Citations
122 Claims
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1-99. -99. (canceled)
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100. A diversity generation device, comprising:
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(i) a programmed thermocycler that receives nucleic acid fragments from (ii) and reassembles them to produce shuffled nucleic acids, wherein the programmed thermocycler comprises a thermocycler operably coupled to a computer, wherein the computer comprises one or more instruction set that does one or more of the following;
calculates an amount of uracil and an amount of thymidine for use in the programmed thermocycler or calculates one or more crossover region between two or more parental nucleic acid sequence; and
(ii) a fragmentation module operably coupled to the programmed thermocycler, wherein the fragmentation module fragments parental nucleic acids. - View Dependent Claims (102, 103, 104, 105, 106, 107, 108, 109, 110, 111)
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101. (canceled)
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112. A diversity generation device comprising:
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(i) a computer, which computer comprises at least a first instruction set for creating nucleic acid fragment sequences from one or more parental nucleic acid sequence, wherein the first instruction set limits or expands diversity encoded by one or more nucleic acid fragment;
(ii) a synthesizer module operably coupled to the computer, which synthesizer module synthesizes the one or more nucleic acid fragment sequence; and
,(iii) a thermocycler operably coupled to the synthesizer, which thermocycler generates diverse sequences from the nucleic acid fragment sequences. - View Dependent Claims (113, 114, 115, 116, 117, 118, 119, 120, 121)
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122-299. -299. (canceled)
Specification