METHOD FOR PRODUCING ALPHA-1, 6-BRANCHED ALPHA-1, 4-GLUCANS FROM SUCROSE
First Claim
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1. An α
- -1,6-branched α
-1,4-glucan, wherein said glucan has a branching degree that is higher than glycogen.
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Abstract
Nucleic acid molecules which encode a branching enzyme from a bacterium of the genus Neisseria, vectors, host cell, plant cells and plants containing said nucleic acid molecules as well as starch obtainable from the plants described are described. Furthermore, an in-vitro method for producing α-1,6-branched α-1,4-glucans on the basis of sucrose and a combination of enzymes of an amylosucrase and a branching enzyme as well as the α-1,6-branched α-1,4-glucans obtainable by said method are described.
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Citations
13 Claims
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1. An α
- -1,6-branched α
-1,4-glucan, wherein said glucan has a branching degree that is higher than glycogen. - View Dependent Claims (2, 3, 4, 5, 6, 13)
- -1,6-branched α
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7. An α
- -1,6-branched α
-1,4-glucan, wherein said glucan has a branching degree that is higher than glycogen, said glucan produced by a method comprising(a) identifying the activity of an amylosucrase and a branching enzyme;
(b) mixing said amylosucrase and said branching enzyme in a ratio of protein activity of 1;
4000-2000;
1;
(c) reacting said mixture of amylosucrase and branching enzyme with a sucrose solution; and
(d) isolating an α
-1,6-branched α
-1,4-glucan with a branching degree that is higher than glycogen. - View Dependent Claims (8, 9, 10, 11, 12)
- -1,6-branched α
Specification