Analytical system based on porous material for highly parallel single cell detection

• US 20080020453A1
• Filed: 07/20/2007
• Published: 01/24/2008
• Est. Priority Date: 07/21/2006
• Status: Abandoned Application

• Current Assignee: Siemens AG
• Original Assignee: Siemens AG
• Inventors: Zilch, Christian, Ehben, Thomas
• Application Number:
  US11/878,088
• Publication Number:
  US 20080020453A1
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2300/045 whereby the whole cover is slidable
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B01L 3/50857 using arrays or bundles of open capillaries for holding samples
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: C12M 47/06 Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: C12M 47/12 Purification C12M47/04 takes precedence

Abstract

At least one embodiment of the present invention relates to analytical systems based on porous material, for example silicon, for highly parallel single cell detection. At least one embodiment of the present invention relates in particular to porous silicon having a multiplicity of continuous channels and/or to the use thereof at least for cell separation, for cell lysis and purification of target molecules, for amplification of nucleic acid molecules or for detection of desired target molecules. At least one embodiment of the present invention also relates to an analytical method using porous silicon. Monoclonal antibodies for cell separation and immobilized capture molecules for cell lysis and purification are attached to the inside walls of the channels.

Claims

1. A flow-through chip made of a porous material and including a multiplicity of continuous channels, a diameter of the channels being between 0.1 and 100 μ

m so that there is space for a single eukaryotic cell and wherein the channels are closeable from a top and bottom of the flow-through chip in a variable manner, but not independently of one another, by movable cover plates, made of at least one of polymeric material and membranes, including a seal arranged between the plates which, with a voltage being applied, serves as a heating element for heating the channels to carry out a polymerase chain reaction, and wherein liquid reagents are passable through the channels, and including at least one of;

monoclonal antibodies, on inside walls of the channels, to separate statistically one desired cell per channel; and

immobilized capture molecules, on inside walls of the channels, to bind at least one of desired cells, desired target molecules, desired target proteins and desired gene sequences.

2. The flow-through chip as claimed in claim 1, wherein the porous material is designed as at least one of a nanotiter plate and a nanotiter chip.

3. The flow-through chip as claimed in claim 1, wherein the porous material is made from at least one of porous glass and porous plastic.

4. The flow-through chip as claimed in claim 1, wherein the porous material is made from porous silicon.

5. The flow-through chip as claimed in claim 4, wherein the porous silicon is in the form of a single crystal plate into which the channels are etched in a continuous manner.

6. A method, comprising:

using a flow-through chip made of porous material, as claimed in claim 1, for at least one of cell separation, cell lysis and purification of target molecules, amplification of nucleic acid molecules and detection of desired target molecules.

9. The flow-through chip as claimed in claim 2, wherein the porous material is made from at least one of porous glass and porous plastic.

10. The flow-through chip as claimed in claim 2, wherein the porous material is made from porous silicon.

11. The flow-through chip as claimed in claim 10, wherein the porous silicon is in the form of a single crystal plate into which the channels are etched in a continuous manner.

12. The flow-through chip as claimed in claim 3, wherein the porous material is made from porous silicon.

13. The flow-through chip as claimed in claim 12, wherein the porous silicon is in the form of a single crystal plate into which the channels are etched in a continuous manner.

7. An analytical method using a flow-through chip made of porous material including a multiplicity of channels with a diameter of between 0.1 and 100 μ

m, comprising at least one of;

a step for cell separation in the channels;

a step for cell lysis and purification of target molecules in the channels;

a step for amplification of nucleic acid molecules in the channels; and

a step for detection of desired target molecules in the channels.

8. The analytical method as claimed in claim 7, wherein at least one of:

the step for cell separation involves applying a histological section to the surface of the porous material and thinning out the cells of the histological section for statistically one cell per channel to be bound;

the step for cell lysis and purification of the target molecules involves arresting the cells present in the channels by way of immobilized capture molecules inside each channel and at least one of lysing the cells biologically, lysing the cells chemically by way of lysis reagents, lysing the cells thermally, lysing the cells by way of ultrasound and lysing the cells mechanically/physically;

the step for amplification of nucleic acid molecules involves heating the channels by way of a heating element to carry out a polymerase chain reaction; and

the step for detection of desired target molecules involves specific capture molecules immobilized to inside walls of the channels interacting with the desired target molecules, subsequently washing unbound labeled molecules out of the channels by pumping through buffer, and then quantifying remaining markers bound via the target molecules with the capture molecules by way of at least one of optical, magnetic, electrochemical and radioactive methods.