Intradermal cellular delivery using narrow gauge micro-cannula

• US 20080021439A1
• Filed: 02/12/2007
• Published: 01/24/2008
• Est. Priority Date: 01/16/2003
• Status: Abandoned Application

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Williams, Dominique, Brittingham, John, Dean, Cheryl, Mikszta, John
• Application Number:
  US11/705,192
• Publication Number:
  US 20080021439A1
• Time in Patent Office: Days
• US Class Current: 604/522
• CPC Class Codes: A61K 2039/54 characterised by the route of administration
• CPC Class Codes: A61K 2239/31 characterized by the route of administration
• CPC Class Codes: A61K 2239/38 characterised by the dose, timing or administration schedule
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 39/0005 Vertebrate antigens from snakes A61K39/38
• CPC Class Codes: A61K 39/461 characterised by the cell type used
• CPC Class Codes: A61K 39/4644 Cancer antigens
• CPC Class Codes: A61M 2037/0061 Methods for using microneedles
• CPC Class Codes: A61M 37/0015 by using microneedles
• CPC Class Codes: A61P 37/04 Immunostimulants

Abstract

A method for delivering cells into a subject by administering cells into the intradermal space of the skin of the subject by a microneedle. The cells are associated with cellular based therapeutics and vaccines and delivered by perpendicular insertion of the microneedle. The microneedle is a hollow needle having an exposed height of between about 0 and 1 mm, a total length of between about 0.3 mm to 2.5 mm, and a size of equal to or less than 30 gauge. An array of microneedles can also be used.

Claims

1. A method for delivering cells to a human or animal, said method comprising administering said cells intradermally through at least one small gauge cannula or needle wherein said cannula or needle is between 30 and 34 gauge, wherein the cells are administered in a concentration of 20-100 million cells/ml.

2. The method of claim 1 wherein said cannula or needle is 30 gauge.

3. The method of claim 1 wherein said cannula or needle is 34 gauge.

4. The method of claim 1 wherein said cells are dendritic cells.

5. The method of claim 4 wherein said cells are interdigitating dendritic cells.

6. The method of claim 4 wherein said cells are immature dendritic cells.

7. The method of claim 4 wherein said cells are mature dendritic cells.

8. The method of claim 1 wherein said cells are delivered into skin at a depth of 0.3 to 2.5 mm.

9. The method of claim 1 wherein said cells are delivered at a flow rate between 100 and 400 μ

l/min.

10. The method of claim 1 wherein said cells are delivered at a flow rate of less than 100 μ

l/min.

11. The method of claim 1 wherein said cells are delivered at a flow rate of greater than 400 μ

l/min.

12. The method of claim 3 wherein said cells are administered at a concentration of less than 80 million cells/ml.

13. The method of claim 3 wherein said cells are administered at a concentration of less than 40 million cells/ml.

14. The method of claim 3 wherein said cells are administered at a concentration of 20 million cells/ml.

15. The method of claim 1 wherein said animal is a mammal.