BIOSENSOR

• US 20080023326A1
• Filed: 08/15/2007
• Published: 01/31/2008
• Est. Priority Date: 05/30/2003
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Zhang, Xiang, McTigue, Catherine, Forrow, Nigel
• Granted Patent: US 7,754,059 B2
• Time in Patent Office: Days
• US Class Current: 204/403.14
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10T 156/10 Methods of surface bonding and/or assembly therefor

Abstract

A biosensor for determining the concentration of an analyte in a biological sample. The biosensor comprises a support, a reference electrode or a counter electrode or both disposed on the support, a working electrode disposed on the support, the working electrode spaced apart from the other electrode or electrodes on the support, a covering layer defining a sample chamber over the electrodes, an aperture in the covering layer for receiving a sample, and at least one layer of mesh in the sample chamber between the covering layer and the electrodes. The at least one layer of mesh has coated thereon a silicone surfactant. Certain silicone surfactants are as effective as fluorinated surfactants with respect to performance of biosensors. These surfactants, when coated onto the mesh layer of the biosensor, are effective in facilitating the transport of aqueous test samples, such as blood, in the sample chamber.

Claims

1-22. -22. (canceled)

23. A biosensor comprising a support, an arrangement of electrodes disposed on the support, a covering layer defining an enclosed space over the electrodes, and at least one mesh layer in the enclosed space between the covering layer and the electrodes, the at least one mesh layer coated with at least one silicone surfactant, and wherein the at least one mesh layer is less than 150 microns in thickness.

24. The biosensor of claim 23, wherein the at least one silicone surfactant comprises a rake-type surfactant.

25. The biosensor of claim 23, wherein the at least one silicone surfactant has the formula:

26. The biosensor of claim 23, comprising a single layer of mesh.

27. The biosensor of claim 23, comprising two layers of mesh.

28. The biosensor of claim 23, wherein the at least one mesh layer comprises a woven or a non-woven material.

29. The biosensor of claim 23, wherein the at least one mesh layer comprises fibers, the fibers comprising a polyamide or a polyester.

30. The biosensor of claim 23, wherein the at least one mesh layer comprises fibers, the fibers having a diameter from about 70 to about 100 microns.

31. The biosensor of claim 23, wherein the at least one mesh layer comprises fibers, the at least one mesh layer having a fiber count of about 40 to about 60 per cm.

32. The biosensor of claim 23, further comprising an aperture in the covering layer for receiving a biological sample, wherein the electrode arrangement comprises a working electrode that includes an enzyme capable of catalyzing a reaction involving a substrate for the enzyme and a mediator capable of transferring electrons between the enzyme-catalyzed reaction and the working electrode to create a current related to the activity of the enzyme and related to a concentration of an analyte in the biological sample.

33. The biosensor of claim 32, wherein the analyte is glucose or a ketone body.

34. A biosensor comprising a support, an arrangement of electrodes disposed on the support, a covering layer defining an enclosed space over the electrodes, and at least one mesh layer in the enclosed space between the covering layer and the electrodes, the at least one mesh layer coated with at least one silicone surfactant, and wherein the at least one mesh layer has an average mesh opening from about 67 to about 180 microns.

35. The biosensor of claim 34, wherein the at least one silicone surfactant comprises a rake-type surfactant.

36. The biosensor of claim 34, comprising a single layer of mesh.

37. The biosensor of claim 34, comprising two layers of mesh.

38. The biosensor of claim 34, wherein the at least one mesh layer has a thickness from about 100 to about 160 microns.

39. The biosensor of claim 34, wherein the at least one mesh layer comprises a woven or a non-woven material.

40. The biosensor of claim 34, wherein the at least one mesh layer comprises fibers, the fibers comprising a polyamide or a polyester.

41. The biosensor of claim 34, wherein the at least one mesh layer comprises fibers, the fibers having a diameter from about 70 to about 100 microns.

42. The biosensor of claim 34, wherein the at least one mesh layer comprises fibers, the at least one mesh layer having a fiber count of about 40 to about 60 per cm.