Biomolecule diagnostic devices and method for producing biomolecule diagnostic devices

• US 20080026454A1
• Filed: 05/07/2007
• Published: 01/31/2008
• Est. Priority Date: 05/03/2002
• Status: Active Grant

• Current Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Original Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Inventors: Kaylor, Rosann, Cohen, David, Sayre, Curtis
• Granted Patent: US 7,695,979 B2
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G03H 1/182 Post-exposure processing, e.g. latensification

Abstract

A biosensor includes a substrate with a layer of receptive material disposed thereon overlying a layer containing a photo-reactive agent. The receptive material is specific for an analyte of interest. A pattern of active and inactive areas of the receptive material are defined in the receptive material layer by a masking process wherein the photo-reactive agent is activated in the exposed regions of the mask.

Claims

1. A biosensor, comprising:

a substrate member;

a photo-reactive agent at a surface of said substrate member;

a receptive material layer applied over said photo-reactive agent and generally uniformly covering said side of said substrate member, said receptive material being specific for an analyte of interest;

a pattern of active and inactive areas of said receptive material defined on said layer, said active and inactive areas formed by a masking process wherein a mask is placed over said substrate member prior to irradiating said substrate member with an energy source sufficient for activating said photo-reactive agent such that areas exposed by said mask define said pattern of inactive areas of said receptive material and said areas shielded by said mask define said pattern of active areas of receptive material; and

wherein when said biosensor is exposed to a medium containing said analyte of interest, the analyte binds to said receptive material in said active areas and subsequently facilitate diffraction of transmitted light or reflected light in a diffraction pattern corresponding to said active areas.

2. The biosensor as in claim 1, wherein said substrate comprises a material from the list of materials consisting of plastics, metal coated plastics and glass, functionalized plastics and glass, silicon wafers, foils, glass, and polymer films.

3. The biosensor as in claim 1, wherein said photo-reactive agent is applied as a layer on said substrate member.

4. The biosensor as in claim 1, wherein said diffraction pattern is visible to the naked eye.

5. The biosensor as in claim 1, wherein said receptive material is protein based.

6. The biosensor as in claim 5, wherein said receptive material is an antibody.

7. The biosensor as in claim 1, wherein said substrate member is irradiated with UV light at a wavelength sufficient for activating said photo-reactive agent exposed through said mask.

8. The biosensor as in claim 1, wherein said receptive material is at least one of an antigen, antibody, nucleotide, chelator, enzyme, bacteria, yeast, fungi, virus, bacterial pili, bacterial flagellar material, nucleic acid, polysaccharide, lipid, protein, carbohydrate, metal, hormone, aptamer, peptide, and respective receptor for said materials.

9. The biosensor as in claim 1, wherein said analyte of interest is at least one of a bacteria, yeast, fungus, virus, rheumatoid factor, IgG, IgM, IgA, IgD, and IgE antibodies, carcinoembryonic antigen, streptococcus Group A antigen, viral antigens, antigens associated with autoimmune disease, allergens, tumor antigens, streptococcus group B antigen, HIV I or HIV II antigen, antibodies viruses, antigens specific to RSV, an antibody, antigen, enzyme, hormone, polysaccharide, protein, lipid, carbohydrate, drug, nucleic acid, Neisseria meningitides groups A, B, C, Y and W sub 135, Streptococcus pneumoniae, E. coli K1, Haemophilus influenza type A/B, an antigen derived from microorganisms, PSA and CRP antigens, a hapten, a drug of abuse, a therapeutic drug, an environmental agents, or antigens specific to Hepatitis.

10. The biosensor as in claim 1, wherein said photo-reactive agent is a photo-acid agent.

11. The biosensor as in claim 1, wherein said photo-reactive agent is a photo-oxidant agent.