Modified Tissue Marking Pigment And Method For Modifying Tissue Marking Pigment

• US 20080028990A1
• Filed: 07/14/2005
• Published: 02/07/2008
• Est. Priority Date: 07/15/2004
• Status: Abandoned Application

• Current Assignee: Freedom-2, Inc.
• Original Assignee: Freedom-2, Inc.
• Inventors: Klitzman, Bruce
• Application Number:
  US11/571,962
• Publication Number:
  US 20080028990A1
• Time in Patent Office: Days
• US Class Current: 106/493
• CPC Class Codes: A61B 2090/395 with marking agent for marking skin or other tissue
• CPC Class Codes: A61K 2800/412 Microsized, i.e. having sizes between 0.1 and 100 microns
• CPC Class Codes: A61K 2800/57 Compounds covalently linked to a(n inert) carrier molecule, e.g. conjugates, pro-fragrances
• CPC Class Codes: A61K 8/11 Encapsulated compositions
• CPC Class Codes: A61K 8/86 Polyethers
• CPC Class Codes: A61Q 1/025 Semi-permanent tattoos, stencils, e.g. "permanent make-up"
• CPC Class Codes: A61Q 19/04 for chemically tanning the skin topical sun or radiation screening or tanning preparations A61Q17/04

Abstract

The invention provides a modified tissue marking pigment and a method for preparing a modified tissue marking pigment by altering protein adsorption to the surface of the pigment particle, activation of key receptors on important immune cells or altering the pigment particle morphology.

Claims

1. A tissue marking pigment comprising a particle having a compound on its surface, which said compound reduces protein adsorption to the surface.

2. The pigment according to claim 1, wherein the compound is a water-soluble polymer.

3. The pigment according to claim 2, wherein the water-soluble polymer is polyethylene glycol, poly anhydride, polyethylene oxide or polyhydroxyethyl methacrylate.

4. The pigment according to claim 3, wherein the polymer is polyethylene glycol.

5. The pigment according to claim 4, wherein a molecular weight of the polyethylene glycol is from greater than about 3,000 Daltons to about 30,000 Daltons.

6. The pigment according to claim 5, wherein the molecular weight of the polyethylene glycol is about 18,500 Daltons.

7. The pigment according to claim 1, wherein a diameter of the particle is less than about 5 μ

m.

8. The pigment according to claim 7, wherein the diameter of the particle is from about 1 μ

m to about 3 μ

m.

9. The pigment according to claim 1, wherein a diameter of the particle is greater than about 15 μ

m.

10. The pigment according to claim 9, wherein the diameter of the particle from about 20 μ

m to about 100 μ

m.

11. The pigment according to claim 1, wherein the compound is covalently or ionically bonded to the surface.

12. The pigment according to claim 1, wherein molecules that interact with specific receptors on a surface of tissue cells or organelles to either suppress or heighten the aggressiveness of an immune system reaction to the particle are on the surface of the particle.

13. The pigment according to claim 12, wherein the molecules are selected from the group consisting of integrins, endotoxin, lipopolysaccharide, tryptophan-arginine containing coating protein and any combination thereof.

14. A tissue marking pigment comprising a particle having a compound on the surface, which said compound increases protein adsorption to the surface.

15. The pigment according to claim 14, wherein molecules that interact with specific receptors on a surface of tissue cells to either suppress or heighten aggressiveness of an immune system reaction to the particle are on the surface of the particle.

16. The pigment according to claim 15, wherein the molecules are selected from the group consisting of integrins, endotoxin, lipopolysaccharide and any combination thereof.

17. The pigment according to claim 14, wherein the compound is covalently or ionically bonded to the surface.

18. The pigment according to claim 14, wherein the compound is a lipopolysaccharide.

19. The pigment according to claim 18, wherein the lipopolysaccharide is obtained from E. Coli or Porphyromonas gingivalis.

20. The pigment according to claim 14, wherein the compound is a cytokine.

21. The pigment according to claim 20, wherein the cytokine is TNF-α

.

22. The pigment according to claim 14, wherein the compound is a leukotriene.

23. The pigment according to claim 22, wherein the leukotriene is LTB4.

24. The pigment according to claim 14, wherein a diameter of the particle is from about 5 μ

m to about 50 μ

m.

25. A method for preparing a tissue marking pigment comprising the steps of:

providing the tissue marking pigment comprising a particle, which has a surface; and

placing a compound on the surface of the particle, which said compound reduces protein adsorption to the surface.

26. The method according to claim 25, wherein the compound is coated on the surface of the particle.

27. The method according to claim 25, wherein the compound is covalently or ionically bonded to the surface of the particle.

28. The method according to claim 25, wherein the compound is a water-soluble polymer.

29. The method according to claim 25, wherein the compound is polyethylene glycol, polyethylene oxide or polyhydroxyethyl methacrylate.

30. The method according to claim 29, wherein the polymer is polyethylene glycol.

31. The method according to claim 30, wherein a molecular weight of said polyethylene glycol is from about 3,000 Daltons to about 30,000 Daltons.

32. The method according to claim 31, wherein the molecular weight of said polyethylene glycol is about 1,500 Daltons.

33. The method according to claim 25, wherein a diameter of the particle is less than about 5 μ

m.

34. The method according to claim 33, wherein the diameter of the particle is from about 1 μ

m to about 3 μ

m.

35. The method according to claim 25, wherein a diameter of the particle is greater than about 15 μ

m.

36. The method according to claim 35, wherein the diameter of the particle is from about 20 μ

m to about 100 μ

m.

37. The method according to claim 25, wherein molecules that interact with specific receptors on a surface of tissue cells to either suppress or heighten aggressiveness of an immune system reaction to the particle are placed on the surface of the particle.

38. The method according to claim 37, wherein the molecules are selected from the group consisting of integrins, endotoxin, lipopolysaccharide and any combination thereof.

39. A method for preparing a tissue marking pigment comprising the steps of:

providing the tissue marking pigment comprising a particle, which has a surface; and

placing a compound on the surface of the particle, which said compound increases protein adsorption to the surface.

40. The method according to claim 39, wherein the compound is coated on the surface of the particle.

41. The method according to claim 39, wherein the compound is covalently or ionically bonded to the surface of the particle.

42. The method according to claim 39, wherein the compound is a lipopolysaccharide.

43. The pigment according to claim 42, wherein the lipopolysaccharide is obtained from E. Coli or Porphyromonas gingivalis.

44. The method according to claim 39, wherein the compound is a cytokine.

45. The method according to claim 44, wherein the cytokine is TNF-α

.

46. The method according to claim 39, wherein the compound is a leukotriene.

47. The method according to claim 46, wherein the leukotriene is LTB4.

48. The method according to claim 39, wherein a diameter of the particle is from about 5 μ

m to about 50 μ

m.

49. The method according to claim 39, wherein molecules that interact with specific receptors on a surface of tissue cells to either suppress or heighten aggressiveness of an immune system reaction to the particle.

50. The method according to claim 49, wherein the molecules are selected from the group consisting of integrins, endotoxin, lipopolysaccharide and any combination thereof.

51. A tissue marking pigment comprising a particle having molecules on its surface, which said molecules interact with specific receptors on a surface of tissue cells to either suppress or heighten aggressiveness of an immune system reaction to the particle.

52. The pigment according to claim 51, wherein the molecules are selected from the group consisting of integrins, endotoxin, lipopolysaccharide and any combination thereof.

53. A tissue marking pigment comprising at least one particle having an outer surface, which comprises at least one projection, at least one recess, at least one indentation and/or at least one pore.

54. The pigment according to claim 53, wherein the outer surface has projections, which are spaced apart by and/or have a length of about 0.1 microns to about 100 microns.

55. The pigment according to claim 53, wherein the outer surface has said at least one pore with a diameter or minor axis of about 0.1 micron to about 100 microns.

56. The pigment according to claim 53, wherein the outer surface has indentations, which are spaced apart by and/or have a length of about 0.1 microns to about 100 microns.

57. A tissue marking pigment comprising at least one particle having an edge with a curvature radius of about 0.1 to about 10 microns.