Enhanced Production of Lipids Containing Polyenoic Fatty Acid by Very High Density Cultures of Eukaryotic Microbes in Fermentors
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Abstract
The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.
123 Citations
102 Claims
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1-84. -84. (canceled)
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85. A process for growing microorganisms of the order Thraustochytriales comprising conducting a fermentation of the microorganisms in a medium comprising a carbon source, and a limiting nutrient source:
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wherein the carbon source and limiting nutrient source are added to the medium to increase the biomass density of the fermentation medium to at least about 100 g/L on a dry cell weight basis;
wherein the fermentation of the medium having a biomass density of at least about 100 g/L on a dry cell weight basis produces lipids containing polyenoic fatty acids; and
wherein the microorganisms are capable of producing at least about 20% of their biomass as lipids comprising some polyenoic fatty acids. - View Dependent Claims (86, 87, 88, 89, 90)
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91. A process for producing microbial lipids comprising:
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growing microorganisms of the order Thraustochytriales in a fermentation medium to increase the biomass density of the fermentation medium;
allowing the microorganisms to produce the lipids when the biomass density of the fermentation medium to at least about 100 g/L on a dry cell weight basis; and
recovering the lipids, wherein greater than about 15% of the lipids are polyunsaturated lipids. - View Dependent Claims (92, 93, 94, 95, 96)
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97. A process for producing microbial lipids comprising conducting a fermentation of microorganisms in a medium comprising a carbon source, and a limiting nutrient source:
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adding carbon source and limiting nutrient source to a fermentation medium to increase the biomass density of the fermentation medium to at least about 100 g/L on a dry cell weight basis;
producing lipids when the fermentation medium has a biomass density of at least about 100 g/L on a dry cell weight; and
recovering the microbial lipids;
wherein at least about 15% of the microbial lipids are polyunsaturated lipids. - View Dependent Claims (98, 99, 100, 101, 102)
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Specification