Production of Cell Culture Product and Material for Use in Said Production
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Accused Products
Abstract
A base plate for use in cell culture which comprises a base material, a water-repellent layer having a water-repellent surface formed on the above base material and a hydrophilic surface having a prescribed pattern formed on the base material; a method for producing the above base plate; and a method for producing a cell culture product using the base plate. It is preferred that the water-repellent surface has a water contact angle of more than 150°. The above method for producing a cell culture product using the base plate base plate is a novel method which allows the production of a cell culture product of a fine pattern.
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Citations
34 Claims
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1-19. -19. (canceled)
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20. A method for producing a cell culture product in a desired pattern, the method comprising:
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preparing a plate for cell culture, the plate having a hydrophilic surface formed in a groove or line pattern of a width of less than or equal to 100 μ
m and a water-repellent surface having a water contact angle of greater than 150°
formed in areas other than the pattern;
disposing protruding droplets of a culture medium selectively along the groove or line pattern on the hydrophilic surface; and
culturing a target cell in the disposed medium. - View Dependent Claims (21, 22, 23, 24, 25, 26)
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27. A method for producing a cell culture product in a desired pattern, the method comprising:
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preparing a plate for cell culture, the plate having a hydrophilic surface formed in a groove or line pattern of a width of less than or equal to 100 μ
m and a water-repellent surface having a water contact angle of greater than 150°
formed in areas other than the pattern; and
culturing a target adherent cell in a state in which the cells are adhered selectively to the hydrophilic surface, wherein protruding droplets of a culture medium are disposed along the groove or line pattern of the hydrophilic surface. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34)
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Specification