Labeling for identification of proteins and other macromolecules

• US 20080032410A1
• Filed: 07/16/2007
• Published: 02/07/2008
• Est. Priority Date: 07/19/2006
• Status: Abandoned Application

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Moerschell, Richard
• Application Number:
  US11/879,280
• Publication Number:
  US 20080032410A1
• Time in Patent Office: Days
• US Class Current: 436/86
• CPC Class Codes: G01N 33/58 involving labelled substances G01N33/53 takes precedence
• CPC Class Codes: G01N 33/583 with non-fluorescent dye label
• CPC Class Codes: G01N 33/6803 General methods of protein analysis not limited to specific proteins or families of proteins

Abstract

A method for identifying one or more proteins or other macromolecules comprises contacting the proteins or other macromolecules or a material that contains them with soluble fluorescent labels and/or Raman labels that bind to at least two different functional groups, side chains or moieties that may be present on or in proteins or other macromolecules, determining the ratio of the two or more functional groups, side chains or moieties, and comparing that ratio with ratios of the said functional groups, side chains or moieties in known proteins or other macromolecules. Kits for carrying out such a process contain fluorescent and/or Raman labels that bind to two or more of said functional groups, side chains or moieties, a database or other medium containing information respecting the ratio of said functional groups, side chains or other moieties in known proteins or other macromolecules, and software for comparing the measured ratio of labels to the predicted ratio of labels to identify the proteins or other macromolecules.

Claims

1. A method for identifying one or more proteins or other macromolecules that comprises contacting the proteins or other macromolecules, or a material that contains them, with two or more soluble fluorescent labels and/or Raman labels that bind to at least two different functional groups, side chains or moieties that may be present on or in proteins or other macromolecules, determining the ratio of the two or more functional groups, side chains or moieties, and comparing that ratio with ratios of the said functional groups, side chains or moieties in known proteins or other macromolecules.

2. A method according to claim 1 in which the labels comprise fluorescent labels.

3. A method according to claim 1 in which the labels comprise Raman labels.

4. A method according to claim 1 in which the one or more proteins or other macromolecules are contacted with labels that bind to at least three different functional groups, side chains or moieties.

5. A method according to claim 1 further comprising determining native fluorescence of amino acids that may be comprised in the one or more proteins or other macromolecules.

6. A kit for identifying one or more proteins or other macromolecules comprising two or more fluorescent labels and/or Raman labels that bind to at least two different functional groups, side chains or moieties that may be present on or in proteins or other macromolecules, a database or other medium containing information respecting the ratio of said functional groups, side chains or moieties in known proteins or other macromolecules, and software for comparing the measured ratio of labels to the predicted ratio of labels to identify the proteins or other macromolecules.

7. A kit according to claim 6 in which the labels comprise fluorescent labels.

8. A kit according to claim 6 in which the labels comprise Raman labels.

9. A kit according to claim 6 in which the labels bind to at least three different functional groups, side chains or moieties.

10. A kit according to claim 6 in which the labels are diluted with other substances that bind to the same functional groups, side chains or moieties but that do not serve a labeling function.