Method for Development of Independent Multivariate Calibration Models
First Claim
1. A method for the development of independent multivariate calibration models comprising the following steps:
- selecting a set of calibration samples with known secondary properties;
measuring the primary properties for each calibration sample on a reference instrument;
converting the measurement results of the primary properties for each calibration sample obtained on the reference instrument to a structure which is equivalent to measurement results for the calibration samples obtained on a calibratable instrument, wherein the conversion is made using correlations of calibration transfer;
comparing the measurement results of the primary properties for each calibration sample measured on the reference instrument which were converted to a structure being equivalent to measurement results obtained on a calibrated instrument with the known secondary properties of the calibration samples;
generating an independent multivariate calibration model by methods of multivariate regression analysis, wherein the independent multivariate calibration model expresses secondary properties of the samples by means of the primary properties measured on the calibrated instrument, characterized in that the mathematical correlations of calibration transfer are determined by;
selecting the set of samples for calibration transfer;
measuring the primary properties of each sample for calibration transfer on both the reference instrument and the calibrated instrument;
comparing the results of measurements of the primary properties of the samples for calibration transfer obtained on the reference instrument with the results of measurements of the primary properties of the same samples obtained on the calibrated instrument, using thereby the methods of multivariate regression analysis;
wherein the independent multivariate calibration model is selected using quantitative parameters of calibration verification.
0 Assignments
0 Petitions
Accused Products
Abstract
A method is provided for development of independent multivariate calibration models used for determination of secondary properties of a sample based on results of measurements of plurality of primary properties of the sample on target instrument. The method allows development of an independent calibration model for the target instrument regarding results of measurements of plurality of primary properties for sufficient amount of calibration samples with known secondary properties at the reference instrument. In this method, a set of transfer samples are selected, and a plurality of measurements of primary properties are made for each transfer sample using both reference and target instruments. Then relationships of transformation are generated from comparison of the target and reference instruments responses to the transfer samples. These relationships capable to perform a multivariate estimations of the target instrument response for each sample from the calibration set based on the reference instrument responses for those samples. The estimated responses of the target instrument for calibration samples are then used to calculate calibration constants in relationships relating estimated primary properties of each calibration sample to known corresponding secondary properties. Thus, an independent calibration model is developed at the target instrument, which allows extending of the calibration sample set and using outlier statistics for detection of outlier samples in the calibration set and for identification of unknown sample that can be analyzed.
38 Citations
12 Claims
-
1. A method for the development of independent multivariate calibration models comprising the following steps:
-
selecting a set of calibration samples with known secondary properties;
measuring the primary properties for each calibration sample on a reference instrument;
converting the measurement results of the primary properties for each calibration sample obtained on the reference instrument to a structure which is equivalent to measurement results for the calibration samples obtained on a calibratable instrument, wherein the conversion is made using correlations of calibration transfer;
comparing the measurement results of the primary properties for each calibration sample measured on the reference instrument which were converted to a structure being equivalent to measurement results obtained on a calibrated instrument with the known secondary properties of the calibration samples;
generating an independent multivariate calibration model by methods of multivariate regression analysis, wherein the independent multivariate calibration model expresses secondary properties of the samples by means of the primary properties measured on the calibrated instrument, characterized in that the mathematical correlations of calibration transfer are determined by;
selecting the set of samples for calibration transfer;
measuring the primary properties of each sample for calibration transfer on both the reference instrument and the calibrated instrument;
comparing the results of measurements of the primary properties of the samples for calibration transfer obtained on the reference instrument with the results of measurements of the primary properties of the same samples obtained on the calibrated instrument, using thereby the methods of multivariate regression analysis;
wherein the independent multivariate calibration model is selected using quantitative parameters of calibration verification. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
Specification