EphA2 BiTE molecules and uses thereof
First Claim
1. A bispecific single chain antibody comprising:
- (a) a first heavy chain variable domain (VH domain) and a first light chain variable domain (VL domain) each from an antibody that immunospecifically binds to CD3, said first VH domain covalently linked to said first VL domain by a first linker of sufficient length such that said first VH domain and said first VL domain fold to form a first binding domain that binds to CD3; and
(b) a second VH domain and a second VL domain from an antibody that immunospecifically binds an epitope of EphA2 exposed on the cell surface, said second VH domain covalently linked to said second VL domain by a second linker of sufficient length such that said second VH domain and said second VL domain fold to form a second binding domain that binds said epitope of EphA2;
wherein said first binding domain and said second binding domain are covalently linked by a third linker of a sufficient length such that said first binding domain and said second binding domain fold independently of each other.
3 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to bispecific single chain antibodies comprising a first binding domain that immunospecifically binds to the T-cell antigen CD3 and a second binding domain that immunospecifically binds to the EphA2 receptor. Such bispecific single chain antibodies are encompassed by the term “EphA2-BiTEs.” The present invention further relates to methods and compositions designed for the treatment, prevention and/or management of disorders associated with aberrant expression and/or activity of EphA2. Such disorders include, but are not limited to, cancer, non-cancer hyperproliferative cell disorders, and infections. The invention further relates to vectors comprising polynucleotides encoding the EphA2-BiTEs of the invention, host cells transformed therewith, and their use in the production of said EphA2-BiTEs. The invention also provides compositions, including pharmaceutical compositions, comprising any of the aforementioned EphA2-BiTEs, polynucleotides or vectors either alone or in combination with one or more prophylactic or therapeutic agents. Also disclosed are methods of screening for said EphA2-BiTEs and kits comprising any of the aforementioned compositions and diagnostic reagents.
-
Citations
33 Claims
-
1. A bispecific single chain antibody comprising:
-
(a) a first heavy chain variable domain (VH domain) and a first light chain variable domain (VL domain) each from an antibody that immunospecifically binds to CD3, said first VH domain covalently linked to said first VL domain by a first linker of sufficient length such that said first VH domain and said first VL domain fold to form a first binding domain that binds to CD3; and
(b) a second VH domain and a second VL domain from an antibody that immunospecifically binds an epitope of EphA2 exposed on the cell surface, said second VH domain covalently linked to said second VL domain by a second linker of sufficient length such that said second VH domain and said second VL domain fold to form a second binding domain that binds said epitope of EphA2;
wherein said first binding domain and said second binding domain are covalently linked by a third linker of a sufficient length such that said first binding domain and said second binding domain fold independently of each other. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 24, 25, 29, 30, 33)
-
-
20-23. -23. (canceled)
-
26-28. -28. (canceled)
-
31-32. -32. (canceled)
Specification