Populations Of Cells And Devices And Systems Including Same
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Abstract
A population of cells is provided. The population of cells comprising at least two subpopulations of cells, wherein a first subpopulation of the at least two subpopulation of cells includes a first reporter expression construct being expressible in a cell of the first subpopulation when exposed to a first analyte and whereas a second subpopulation of the at least two subpopulation of cells includes a second reporter expression construct being expressible in a cell of the second subpopulation when exposed to a second analyte.
15 Citations
74 Claims
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1-55. -55. (canceled)
- 56. A population of cells comprising at least two subpopulations of cells, wherein a first subpopulation of said at least two subpopulation of cells includes a first reporter expression construct being expressible in a cell of said first subpopulation when exposed to a first analyte and whereas a second subpopulation of said at least two subpopulation of cells includes a second reporter expression construct being expressible in a cell of said second subpopulation when exposed to a second analyte.
- 62. A device for detecting presence, absence or level of a substance in a sample, the device comprising a substrate being configured for supporting a population of cells including at least two subpopulations of cells, wherein a first subpopulation of said at least two subpopulations of cells includes a first reporter expression construct being expressible in a cell of said first subpopulation when exposed to a first analyte and whereas a second subpopulation of said at least two subpopulation of cells includes a second reporter expression construct being expressible in a cell of said second subpopulation when exposed to a second analyte, wherein each of said at least two subpopulations of cells is attached to said substrate in an addressable manner.
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66. A system for detecting presence, absence or level of a substance in a sample, the system comprising:
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(a) a device including a substrate being configured for supporting a population of cells including at least two subpopulations of cells, wherein a first subpopulation of said at least two subpopulations of cells includes a first reporter expression construct being expressible in a cell of said first subpopulation when exposed to a first analyte and whereas a second subpopulation of said at least two subpopulation of cells includes a second reporter expression construct being expressible in a cell of said second subpopulation when exposed to a second analyte, wherein each of said at least two subpopulations of cells is attached to said substrate in an addressable manner; (b) a detector for detecting expression from each of said first and second reporter expression constructs in said population of cells; and (c) a processing unit for obtaining and processing data representing said expression detected by said detector to thereby provide information relating to the presence, absence or level of the substance in the sample.
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67. A method of detecting presence, absence or level of a substance in a sample the method comprising:
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(a) exposing a population of cells to the sample, said population of cells including at least two subpopulations of cells, wherein a first subpopulation of said at least two subpopulations of cells includes a first reporter expression construct being expressible in a cell of said first subpopulation when exposed to a first analyte and whereas a second subpopulation of said at least two subpopulation of cells includes a second reporter expression construct being expressible in a cell of said second subpopulation when exposed to a second analyte with the sample; and (b) analyzing expression of said reporter expression constructs in each of said at least two subpopulations of cells, to thereby detect presence, absence or level of the substance in the sample. - View Dependent Claims (68, 69, 70, 71, 72, 73, 74)
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Specification