Placental niche and use thereof to culture stem cells
First Claim
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1. A method of culturing a stem cell comprising culturing the stem cell in a culture medium with a collagen biofabric, wherein said collagen biofabric is derived from a placenta, and said stem cell is exogenous to said collagen biofabric.
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Abstract
The present invention provides methods for culturing, expanding and differentiating stem cells, particularly human embryonic stem cells. The methods comprise culturing the stem cells for a period of time on a collagen biofabric, particularly a collagen biofabric derived from the amniotic membrane, chorion, or both, from mammalian placenta.
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Citations
37 Claims
- 1. A method of culturing a stem cell comprising culturing the stem cell in a culture medium with a collagen biofabric, wherein said collagen biofabric is derived from a placenta, and said stem cell is exogenous to said collagen biofabric.
- 19. A method of differentiating a stem cell comprising culturing said cell in culture medium with a collagen biofabric for a time sufficient for differentiation of the cell.
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34. A method of determining the toxicity of a compound to a cell, comprising culturing said cell with a collagen biofabric under conditions suitable for the survival of the cell;
- contacting said cell with the compound; and
identifying a change in a metabolic parameter of said cell indicating apoptosis, necrosis, or cell death, or a tendency towards apoptosis, necrosis or cell death, as compared to a cell cultured under equivalent conditions and not contacted with said compound, wherein if said change is identified, said compound is toxic to said cell.
- contacting said cell with the compound; and
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35. A method of culturing a stem cell, comprising culturing the stem cell with a collagen biofabric comprising a plurality of placental stem cells;
- and culturing said stem cell under conditions appropriate for the survival of the stem cell.
Specification