Nano-Chem-Fet Based Biosensors

• US 20080044911A1
• Filed: 10/18/2007
• Published: 02/21/2008
• Est. Priority Date: 10/10/2002
• Status: Active Grant

• Current Assignee: Shoei Chemical Inc.
• Original Assignee: Nanosys Incorporated (Shoei Electronic Materials, Inc.)
• Inventors: Daniels, R., Owicki, John, Empedocles, Stephen, Bock, Larry
• Granted Patent: US 7,888,292 B2
• Time in Patent Office: Days
• US Class Current: 436/63
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: G01N 27/4146 involving nanosized elements, e.g. nanotubes, nanowires
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

Methods of detecting a component of interest, a change in charge, a pH, a cellular response using nanosensors are provided. Nanosensors, including nanowires and nanowire arrays comprising functionalized and/or non-functionalized nanowires are provided. Nanosensors are used for detection in cellular fragmentation, multiple concentration analysis, glucose detection, and intracellular analysis.

Claims

1. A method of detecting glucose, the method comprising:

(a) providing one or more nanowires that comprise glucose oxidase immobilized thereon or proximal thereto;

(b) contacting the nanowires with a test solution;

wherein any glucose present in the test solution is oxidized by the glucose oxidase resulting in a change in pH of the test solution, wherein the change in pH produces a signal in the nanowires; and

,

(c) detecting the signal, thereby detecting the glucose in the test solution.

2. The method of claim 1, wherein the test solution comprises one or more cells, one or more cellular fragments, a tissue sample, a cell lysate, or a blood sample.

3. The method of claim 1, wherein the one or more nanowires are positioned in one or more microwells or within a microfluidic device.

4. The method of claim 1, wherein the one or more nanowires comprise a nanowire array.