Novel FRT recombination sites and methods of use
First Claim
1. An isolated polynucleotide comprising a nucleotide sequence comprising at least a first FRT recombination site, the first FRT recombination site comprises a spacer sequence selected from the group consisting of SEQ ID NOS:
- 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, and 18.
0 Assignments
0 Petitions
Accused Products
Abstract
Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
-
Citations
56 Claims
-
1. An isolated polynucleotide comprising a nucleotide sequence comprising at least a first FRT recombination site, the first FRT recombination site comprises a spacer sequence selected from the group consisting of SEQ ID NOS:
- 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, and 18.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
-
20. A method for producing site-specific recombination of DNA comprising
a) providing a first DNA comprising a first site-specific recombination site, wherein the first site-specific recombination site comprises a polynucleotide selected from the group consisting of: -
i) an FRT recombination site comprising a spacer region selected from the group consisting of SEQ ID NOS;
1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, and 18; and
,ii) an FRT recombination site selected from the group consisting of 21, 22, 23, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, and 38;
b) providing a second DNA comprising a second site-specific recombination site which is recombinogenic with the first site-specific recombination site; and
,c) providing a site-specific recombinase that catalyzes a site-specific recombination between the first and the second site-specific recombination sites. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
-
-
53. An isolated library comprising a population of plasmids wherein each plasmid in the population comprises a first common selectable marker;
- and each plasmid in the population comprises a member of a population of modified FRT recombination sites, wherein each member of the population of modified FRT recombination sites comprises a spacer region comprising at least one nucleotide alteration in SEQ ID NO;
43, wherein the population of plasmids comprises at least about 100 distinct members of the plasmid population. - View Dependent Claims (54, 55, 56)
- and each plasmid in the population comprises a member of a population of modified FRT recombination sites, wherein each member of the population of modified FRT recombination sites comprises a spacer region comprising at least one nucleotide alteration in SEQ ID NO;
Specification