Integrated portable biological detection system
First Claim
1. An integrated system for the analysis of eukaroytic and/or prokaryotic cells in a biological sample comprising:
- an electronic system for cell separation, cell lysis, sample preparation, and sample analysis further comprising a flow cell, an input port coupled to the flow cell, an output port coupled to the flow cell, a plurality of individually addressable electrodes positioned within the flow cell and coupled to a power source configured to electronically disrupt cell membranes within the sample, and an array of probes coupled to the electrodes and adapted to bind to predetermined components within the sample for analysis of the components, and a heating element coupled to the flow cell, wherein the flow cell is adapted to receive the sample via the input;
a detector operatively positioned to detect the molecules bound to the probes by a detectable signal;
a power source coupled to the flow cell; and
a portable housing configured to contain the electronic system, the detector, and the power source.
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Accused Products
Abstract
We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
102 Citations
1 Claim
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1. An integrated system for the analysis of eukaroytic and/or prokaryotic cells in a biological sample comprising:
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an electronic system for cell separation, cell lysis, sample preparation, and sample analysis further comprising a flow cell, an input port coupled to the flow cell, an output port coupled to the flow cell, a plurality of individually addressable electrodes positioned within the flow cell and coupled to a power source configured to electronically disrupt cell membranes within the sample, and an array of probes coupled to the electrodes and adapted to bind to predetermined components within the sample for analysis of the components, and a heating element coupled to the flow cell, wherein the flow cell is adapted to receive the sample via the input;
a detector operatively positioned to detect the molecules bound to the probes by a detectable signal;
a power source coupled to the flow cell; and
a portable housing configured to contain the electronic system, the detector, and the power source.
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Specification