Articles having localized molecules disposed thereon and methods of producing and using same
First Claim
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1. A substrate, comprising:
- a polymerization complex attached to a surface of the substrate, the complex comprising a nucleic acid polymerase enzyme, a target nucleic acid sequence and a nascent nucleic acid sequence synthesized by the polymerase with the target nucleic acid sequence as a template, wherein the nascent nucleic acid sequence is at least 100 bases in length.
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Abstract
Sequencing methods and compositions, substrates, devices and systems are provided. Methods include synthesizing a nascent nucleic acid sequence that is greater than 100 bases in length and sequencing the nucleic acids by detecting synthesis. Compositions and substrates that include polymerization complexes for the methods are included.
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Citations
20 Claims
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1. A substrate, comprising:
a polymerization complex attached to a surface of the substrate, the complex comprising a nucleic acid polymerase enzyme, a target nucleic acid sequence and a nascent nucleic acid sequence synthesized by the polymerase with the target nucleic acid sequence as a template, wherein the nascent nucleic acid sequence is at least 100 bases in length. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of determining a sequence of nucleic acids in a target nucleic acid sequence, comprising:
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attaching a polymerization complex to a surface of a substrate, the polymerization complex comprising a nucleic acid polymerase enzyme, the target nucleic acid sequence and a primer sequence complementary to at least a portion of the target nucleic acid sequence;
providing four different nucleotide analogs having fluorescent labels attached thereto, to the complex to allow target dependent extension of the primer sequence;
synthesizing a nascent nucleic acid sequence that is greater than 100 bases in length; and
detecting incorporation of the nucleotide analogs incorporated during the synthesizing step. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification