Method for Extracting Nucleic Acids

• US 20080051572A1
• Filed: 10/01/2007
• Published: 02/28/2008
• Est. Priority Date: 12/31/2001
• Status: Abandoned Application

• Current Assignee: Industrial Technology Research Institute
• Original Assignee: Industrial Technology Research Institute
• Inventors: HUANG, Shr-Hau, LIN, Ju-Hwa, CHUNG, Yung-Chiang, JAN, Ming-Shiung
• Application Number:
  US11/865,255
• Publication Number:
  US 20080051572A1
• Time in Patent Office: Days
• US Class Current: 536/25.41
• CPC Class Codes: B01F 2215/0422 Numerical values of angles
• CPC Class Codes: B01F 2215/0431 Numerical size values, e.g. diameter of a hole or conduit, area, volume, length, width, or ratios thereof
• CPC Class Codes: B01F 2215/045 Numerical flow-rate values
• CPC Class Codes: B01F 25/104 characterised by the arrangement of the discharge opening
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00783 Laminate assemblies, i.e. the reactor comprising a stack of plates
• CPC Class Codes: B01J 2219/00822 Metal
• CPC Class Codes: B01J 2219/00828 Silicon wafers or plates
• CPC Class Codes: B01J 2219/00889 Mixing
• CPC Class Codes: B01J 2219/00921 by absorption

Abstract

A device for extracting nucleic acids includes a cover including a first opening and a second opening spaced apart from the first opening, a chip including a first terminal to communicate with the first opening and a second terminal to communicate with the second opening, and a flow channel of the chip including an inner surface onto which immobilized beads are dispersed for binding with nucleic acids in a fluid flowing in the flow channel.

Claims

1. A method for extracting nucleic acids, comprising:

providing a chip;

providing the chip with a flow channel including an inner surface; and

dispersing immobilized beads for extracting the nucleic acids on the inner surface of the flow channel.

2. The method of claim 1, further comprising:

adding bead powder in deionized water;

injecting a solution of the bead powder and deionized water into the flow channel; and

shaking the solution in the flow channel.

3. The method of claim 1, further comprising flowing a fluid in the flow channel bi-directionally.

4. The method of claim 1, further comprising providing the flow channel with projecting portions formed into the chip.

5. The method of claim 1, further comprising mixing a sample and a buffer in a swirl before injecting the sample and buffer into the flow channel.

6. A method for extracting nucleic acids, comprising:

providing a flow channel;

providing a solution of bead powder and deionized water;

injecting the solution of bead powder and deionized water into the flow channel; and

shaking the solution in the flow channel to disperse immobilized beads in the flow channel.

7. The method of claim 6, further comprising flowing a fluid in the flow channel bi-directionally.

8. The method of claim 6, further comprising providing the flow channel with projecting portions.

9. The method of claim 6, further comprising generating a swirl of a fluid before injecting the fluid into the flow channel.

10. The method of claim 6, further comprising eluting the immobilized beads by deionized water to release the nucleic acids.