METHOD AND APPARATUS FOR BIOSENSOR SPECTRAL SHIFT DETECTION
First Claim
1. A method for determining biosensor resonance peak offsets comprising the steps:
- determining a reference spectrum;
measuring a sample spectrum from a biosensor;
correlating the reference spectrum and the sampled spectrum;
determining an offset in response to a maximum correlation value.
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Abstract
Performing high-resolution determination of the relative shift of the spectral properties of a biosensor. The shift in the resonance peak of the biosensor is indicative of the amount of material bound to the surface of the biosensor. A preferred biosensor is a Guided Mode Resonant Filter Biosensor (GMRFB). In one aspect of the invention, curve fitting is used to determine the relative location of the spectrum of the unexposed biosensor with respect to those spectra that are altered (e.g., shifted) by the presence of materials bound to the surface of the biosensor. In an alternative embodiment, the cross correlation function is used to detect spectral peak offsets between a reference spectrum and a spectrum measured from an exposed biosensor. In yet another alternative, maximal likelihood estimation techniques are used to determine the spectral shift or offs.
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Citations
25 Claims
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1. A method for determining biosensor resonance peak offsets comprising the steps:
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determining a reference spectrum;
measuring a sample spectrum from a biosensor;
correlating the reference spectrum and the sampled spectrum;
determining an offset in response to a maximum correlation value. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for determining biosensor resonance peak offsets comprising the steps:
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determining reference band edges of a biosensor reference spectrum;
measuring a sample spectrum from a biosensor;
determining sample band edges of the sample spectrum;
determining an offset in response to reference band edges and the sample band edges. - View Dependent Claims (8, 9, 10, 11)
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12. A method for determining biosensor resonance peak offsets comprising the steps:
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(i) determining a reference spectrum and a sample spectrum;
(ii) determining an initial offset estimate;
(iii) determining an incremental offset value;
(iv) updating the initial offset estimate in response to the incremental offset value. - View Dependent Claims (13, 14, 15, 16, 17)
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18. A method for determining biosensor resonance peak offsets comprising the steps:
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(i) providing a peak value of a reference spectrum;
(ii) measuring a sample spectrum;
(iii) determining a peak value of the sample spectrum by fitting a curve to the measured sample spectrum;
(iv) determining a spectral resonance offset in response to the peak value of a reference spectrum and the peak value of the sample spectrum. - View Dependent Claims (19, 20, 21)
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22. A method for determining biosensor resonance peak offsets comprising the steps:
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(i) determining a reference spectrum and a sample spectrum;
(ii) performing an iterative calculation to estimate spectral offset of the sample spectrum based on a maximal likelihood estimation, wherein each iteration is performed in response to the signal amplitude values of the reference spectrum and the sample spectrum and signal slope values of at least one of the reference spectrum and the measured spectrum. - View Dependent Claims (23, 24)
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25-27. -27. (canceled)
Specification