Target for Laser Desorption/Ionisation Mass Spectrometry

• US 20080054171A1
• Filed: 04/04/2004
• Published: 03/06/2008
• Est. Priority Date: 04/02/2004
• Status: Abandoned Application

• Current Assignee: Guenther Bonn
• Original Assignee: Gunther Bonn, Physikalisches BURO Steinmuller GmbH
• Inventors: Stecher, Gunther, Najam-Al-Haq, Muhammad, Schwarzmann, Georg, Huck, Christian, Feuerstein, Isabel, Bonn, Gunther, Steinmuller, Detlef, Steinmuller-Nethl, Doris, Rainer, Matthias
• Application Number:
  US11/547,411
• Publication Number:
  US 20080054171A1
• Time in Patent Office: Days
• US Class Current: 250/282
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: H01J 49/0418 for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates

Abstract

The invention relates to a target for a laser desorption/ionisation mass spectrometer, comprising a substrate that is at least partially coated with a carbon-containing layer comprising a material selected from the group consisting of diamond, amorphous carbon, DLC (diamond-like carbon), graphite, nanotubes, nanowires, fullerenes and mixtures thereof.

Claims

1-35. -35. (canceled)

36. A target for a laser desorption/ionization mass spectrometer, comprising a substrate, wherein said substrate is at least partially coated with a carbon-containing layer comprising a material selected from the group consisting of diamond, amorphous carbon, DLC (diamond-like carbon), graphite, nanotubes, nanowires, fullerenes and mixtures thereof.

37. The target of claim 36, wherein said carbon-containing layer comprises nanocrystalline, polycrystalline, ultrananocrystalline or monocrystalline diamonds.

38. The target of claim 36, wherein said carbon-containing layer has a diamond crystallite portion of at least 10%.

39. The target of claim 37, wherein said diamonds of said carbon-containing layer have a crystallite size from about 0.1 to 500 nm.

40. The target of claim 36, wherein said carbon-containing layer has a thickness of about 0.1 nm to 50 μ

m.

41. The target of claim 36, wherein said carbon-containing layer is electrically conductive.

42. The target of claim 36, wherein said substrate is electrically conductive.

43. The target of claim 36, wherein said carbon-containing layer is modified to be electrically conductive.

44. The target of claim 43, wherein said modification of said carbon-containing layer is electrically conductive by interaction with adsorbed substances or doping.

45. The target of claim 36, wherein said substrate comprises a material selected from the group consisting of graphite, titanium, metal, metal oxides, mineral oxides, semiconductors, polymer, plastic, ceramics, glass, quartz glass, silica gel, steel, composite materials, nanotubes, nanowires, fullerenes and mixtures thereof.

46. The target of claim 36, wherein said carbon-containing layer has hydrophilic and hydrophobic regions.

47. The target of claim 36, wherein said carbon-containing layer is chemo-physically modified.

48. The target of claim 47, wherein said chemo-physically modified carbon-containing layer has at least one binding functionality selected from the group consisting of polar groups, apolar groups, ionic groups, groups having affinity, specific groups, metal-complexing groups and mixtures thereof.

49. The target of claim 36 wherein said carbon-containing layer further comprises a chemical modification wherein said modification includes additional substances selected from the group consisting of hydrogen atoms, halogens, halogen compounds, hydroxyl groups, carbonyl groups, aromatic ring systems, sulfur, sulfur derivatives, Grignard compounds, amino groups, epoxides, metals or carbon chains.

50. The target of claim 36 wherein said carbon-containing layer further comprises at least one binding functionality selected from the group consisting of carbon double bonds, epoxides, halogens, halogen compounds, amino groups, hydroxy groups, acid groups, acid chlorides, cyanide groups, aldehyde groups, sulfate groups, sulfonate groups, phosphate groups, metal-complexing groups, thioethers, biotin, thiolene and mixtures thereof.

51. The target of claim 36 wherein said carbon-containing layer further comprises a chemical modification with at least one linker.

52. The target of claim 51, wherein said linker comprises at least one binding functionality.

53. The target of claim 52, wherein said binding functionality is selected from the group consisting of carbon bonds, epoxides, halogens, halogen compounds, amino groups, hydroxy groups, acid groups, acid chlorides, cyanide groups, aldehyde groups, sulfate groups, sulfonate groups, phosphate groups, metal-complexing groups, thioethers, biotin, thiolene and mixtures thereof.

54. The target of claim 51, wherein said linker comprises an epoxide group selected from the group consisting of glycidyl methacrylate, 3,4-epoxybutyl acrylate, 2-methyl-2-propenyl-oxiranecarboxylic ester, methyl 3-(2-methyloxiranyl)-2-propenoate, dihydro-4-(2-propenyloxy)-2(3H)-furanone, oxiranylmethyl 2-methyl-2-propenoate, tetrahydro-3-furanyl-2-propenoic ester, oxiranylmethyl-2-butenoic ester, 1-methylethenyl-oxirane acetic ester, oxiranylmethyl-3-butenoic ester, (3-methyloxiranyl)methyl-2-propenoic ester, ethyl 3-oxiranyl-2-propenoate, 2-methyl-2-propenyl-oxirane carboxylic ester, 2-oxiranylethyl-2-propenoic ester, 3-(3-butenyl)oxirane carboxylic acid, allyl 2,3-epoxy-buttyric ester, 2,3-epoxypropyl-crotonic ester, tetrahydro-2-furanyl-2-propenoic ester, (2-methyloxiranyl)methyl-2-propenoic ester, 3-oxetanyl 2-methyl-2-propenoate, and mixtures thereof.

55. The target of claim 51, wherein said linker comprises a chemical group selected from the group consisting of iminodiacetic acid, nitrilotriacetic acid, N-carboxy-α

-alanine, aspartic acid, 2-amino-2-methyl-propanedioic acid, 2-β

-ranacetic acid, 5-ethyl-3-hydroxy-4-methyl-2(5H)-furanone, tetrahydro-4-methylene-3-furanacetic acid, aspartic acid, 2-butenedioic acid, methylene-propanedioic acid and mixtures thereof.

56. The target of claim 51, wherein said linker includes an amino group selected from the group consisting of 10-undecene-1-amine, 1-amino-5-hexene, N-2-propenyl-2,2,2-trifluoroacetamide and mixtures thereof.

57. The target of claim 51, wherein said linker includes a carboxylic acid group and is preferably selected from the group consisting of 2-butenedioic acid, ethylenedicarboxylic acid and mixtures thereof.

58. The target of claim 51, wherein said linker includes a halogen compound selected from the group consisting of propenyl chloride, butenyl chloride, 1-bromopropene, 1-chloropropene, 2-bromopropene, 2-chloropropene, 4-chloro-1-butene, 4-chloro-2-butene, 3-chloro-1-butene, 2-methyl-1-chloro-1-propene, 1-chloro-2-butene, 1-chloro-1-butene, 2-chloro-3-methyl-2-butene, 3-chloro-2-methyl-2-butene, 4-chloro-2-pentene, 2-chloro-2-pentene, 1-chloro-1-pentene, 1-chloro-3-methyl-1-butene, 1-chloro-2-methyl-1-butene, 3-chloro-2-pentene, 5-chloro-2-pentene, 1,5-dichloro-2-pentene, 4,4-dichloro-2-methyl-1-butene, 2-chloro-5-methyl-3-hexene, 3-chloro-4-methyl-1-hexene, 2-chloro-2-methyl-3-hexene and mixtures thereof.

59. The target of claim 36, wherein said substrate consists of graphite or titanium coated additional to said carbon-containing layer.

60. The target of claim 36, wherein said carbon-containing layer has a matrix adsorbed or covalently bound to said carbon-containing layer.

61. The target of claim 36, wherein said target has been applied in a replaceable manner to a target holder.

62. The target holder of claim 61, wherein said target holder can be placed directly into the mass spectrometer.

63. The use of a target of claim 36 for examining at least one sample by matrix-assisted laser desorption/ionization mass spectrometry or surface-enhanced laser desorption/ionization mass spectrometry.

64. A process for analyzing a sample by means of surface-enhanced laser desorption/ionization mass spectrometry, comprising:

(a) combining a sample with a target of claim 36;

(b) optionally removing the substances not bound to the target;

(c) optionally adding a matrix, embedding the sample in said matrix by means of evaporating a solvent; and

(d) analyzing said sample combined with a target by means of mass spectrometry.

65. A process for analyzing a sample by means of matrix-assisted laser desorption/ionization mass spectrometry, comprising:

(a) combining sample with a target of claim 36;

(b) optionally removing the substances not bound to the target;

(c) optionally adding a matrix, embedding the sample in said matrix by means of evaporating a solvent; and

(d) analyzing said sample combined with a target by means of mass spectrometry.

66. A carbon-containing particle or bead comprising a carbon-containing layer as defined by claim 36.

67. The use of carbon-containing particles or bead as claimed in claim 66 for selectively binding at least one analyte of a sample and for subsequently analyzing the loaded particles and/or the analytes eluted from said particle or bead by means of laser desorption/ionization mass spectrometry.

68. A carbon-containing powder comprising a carbon-containing layer material as defined by claim 36.

69. The use of a carbon-containing powder as claimed in claim 68 for selectively binding one or more analytes of a sample and subsequently analyzing the loaded powder or the analytes eluted from said powder by means of laser desorption/ionization mass spectrometry.

70. A paste-like mass comprising a carbon-containing material as defined in claim 36.

71. The use of a paste-like mass as claimed in claim 70 for binding one or more analytes of a sample and subsequently analyzing the loaded mass or the analytes eluted from said mass by means of laser desorption/ionization mass spectrometry.