CHIP AND CARTRIDGE DESIGN CONFIGURATION FOR PERFORMING MICRO-FLUIDIC ASSAYS
First Claim
1. An assembly for performing micro-fluidic assays comprising:
- a micro-fluidic chip having a top side and a bottom side and including;
one or more access ports formed in said top side; and
at least one micro-channel extending from an associated access port through at least a portion of said micro-fluidic chip, whereby each access port communicates with an associated micro-channel, such that fluid dispensed into said access port will flow into the associated micro-channel; and
a fluid cartridge having one or more internal chambers for containing fluids and a fluid nozzle associated with each internal chamber for dispensing fluid from the associated chamber or transmitting fluid into the associated internal chamber, each fluid nozzle being configured to be coupled to an access port of said micro-fluidic chip to thereby dispense fluid from the associated internal chamber into the access port with which the nozzle is coupled or to transmit fluid from the access port with which the nozzle is coupled into the associated internal chamber.
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Accused Products
Abstract
An assembly for performing micro-fluidic assays includes a micro-fluidic chip with access ports and micro-channels in communication with the access ports and a fluid cartridge having internal, fluid-containable chambers and a nozzle associated with each internal chamber that is configured to be coupled with an access port. Reaction fluids, such as sample material, buffer, and/or reagent, contained within the cartridge are dispensed from the cartridge into the access ports and micro-channels of the micro-fluidic chip. Embodiments of the invention include a cartridge which includes a waste compartment for receiving used DNA and other reaction fluids from the micro-channel at the conclusion of the assay.
264 Citations
25 Claims
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1. An assembly for performing micro-fluidic assays comprising:
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a micro-fluidic chip having a top side and a bottom side and including; one or more access ports formed in said top side; and at least one micro-channel extending from an associated access port through at least a portion of said micro-fluidic chip, whereby each access port communicates with an associated micro-channel, such that fluid dispensed into said access port will flow into the associated micro-channel; and a fluid cartridge having one or more internal chambers for containing fluids and a fluid nozzle associated with each internal chamber for dispensing fluid from the associated chamber or transmitting fluid into the associated internal chamber, each fluid nozzle being configured to be coupled to an access port of said micro-fluidic chip to thereby dispense fluid from the associated internal chamber into the access port with which the nozzle is coupled or to transmit fluid from the access port with which the nozzle is coupled into the associated internal chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A cartridge device configured to interface with a micro-fluidic chip comprising:
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a delivery chamber in fluid communication with a delivery port, wherein said delivery chamber is configured to contain a reaction fluid and said delivery port is configured to interface with a micro-fluidic chip; and a recovery chamber in fluid communication with a recovery port, wherein said recovery chamber is configured to receive waste materials from said micro-fluidic chip and said recovery port is configured to interface with said micro-fluidic chip. - View Dependent Claims (19, 20)
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21. A cartridge device configured to interface to a micro-fluidic chip comprising:
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a reagent delivery chamber, wherein the reagent delivery chamber is connected to a reagent delivery port; a buffer delivery chamber, wherein the buffer delivery chamber is connected to a buffer delivery port; a sample delivery chamber, wherein the sample delivery chamber is connected to a sample delivery port; a waste recovery chamber, wherein the waste recovery chamber is connected to a waste recovery port; and wherein said reagent delivery port, said buffer delivery port, said sample delivery port and said waste recovery port are configured to interface with the micro-fluidic chip. - View Dependent Claims (22, 23, 24)
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25. A micro-fluidic chip for DNA analysis applications whereby via negative pressure control, DNA samples are introduced via a cartridge and PCR reagents are introduced through a sipper tube that connects to a micro well plate.
Specification