Detection of hydrogen peroxide released by enzyme-catalyzed oxidation of an analyte
First Claim
1. A diagnostic test kit for detecting hydrogen peroxide released by enzyme-catalyzed oxidation of an analyte, the test kit comprising a lateral flow device that contains a chromatographic medium, wherein the chromatographic medium defines a detection zone within which a chromogen is substantially non-diffusively immobilized in conjunction with an anionic compound, the chromogen being a leuco base or a derivative thereof that is capable of undergoing a detectable color change upon oxidation.
1 Assignment
0 Petitions
Accused Products
Abstract
A diagnostic test kit for detecting hydrogen peroxide released by enzyme-catalyzed oxidation of an analyte within a test sample is provided. The test kit comprises a lateral flow device that contains a chromatographic medium, wherein the chromatographic medium defines a detection zone within which a chromogen is substantially non-diffusively immobilized in conjunction with an anionic compound. The chromogen is a leuco base, or a derivative thereof, which is capable of undergoing a detectable color change upon oxidation.
-
Citations
32 Claims
- 1. A diagnostic test kit for detecting hydrogen peroxide released by enzyme-catalyzed oxidation of an analyte, the test kit comprising a lateral flow device that contains a chromatographic medium, wherein the chromatographic medium defines a detection zone within which a chromogen is substantially non-diffusively immobilized in conjunction with an anionic compound, the chromogen being a leuco base or a derivative thereof that is capable of undergoing a detectable color change upon oxidation.
-
19. A method for detecting hydrogen peroxide released by enzyme-catalyzed oxidation of an analyte, the method comprising:
-
providing a lateral flow device that comprises a chromatographic medium, the chromatographic medium defining a detection zone within which an oxidizable chromogen is substantially non-diffusively immobilized in conjunction with an anionic compound, wherein the oxidizable chromogen is a leuco base or a derivative thereof; reacting the hydrogen peroxide with an electron donor to form an intermediate compound; allowing the intermediate compound to flow through the chromatographic medium and contact the detection zone, whereby the intermediate compound oxidizes the chromogen and induces a color change, the oxidized chromogen having a color that differs from the color of the oxidizable chromogen; and detecting the color of the oxidized chromogen. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
-
Specification