PROCESS FOR ENZYMATIC HYDROLYSIS OF PRETREATED LIGNOCELLULOSIC FEEDSTOCKS
First Claim
1. A process for enzymatic hydrolysis of a pretreated lignocellulosic feedstock to soluble sugars, the process comprising adding a cellulase enzyme mixture to the pretreated lignocellulosic feedstock, said cellulase enzyme mixture comprising a primary cellulase mixture of CBH1 and CBH2 cellobiohydrolases and EG1 and EG2 endoglucanases, said CBH1 and CBH2 cellobiohydrolases being present at greater than or equal to 55% and less than 85% of the primary cellulase mixture, said CBH2 being present at a fraction relative to CBH1 and CBH2 cellobiohydrolases as defined by fC2 and said EG2 being present at a fraction relative to the EG1 and EG2 endoglucanases as defined by fE2, wherein when said CBH1 and CBH2 cellobiohydrolases are present at greater than or equal to 55% and less than 65% of the primary cellulases, said fC2 values are greater than or equal to 0.35 and less than 0.45 and said fE2 values are greater than or equal to 0.15 and less than 0.35;
- said fC2 values are greater than or equal to 0.45 and less than 0.55 and said fE2 values greater than or equal to 0.15 and less than 0.25;
or said fC2 values are greater than or equal to 0.45 and less than 0.55 and said fE2 values are greater than or equal to 0.35 and less than 0.45, when said CBH1 and CBH2 cellobiohydrolases are present at greater than or equal to 65% and less than 75% of the primary cellulases, said fC2 values are greater than or equal to 0.25 and less than 0.35 and said fE2 values are greater than or equal to 0.05 and less than 0.35;
said fC2 values are greater than or equal to 0.35 and less than 0.45 and said fE2 values are greater than or equal to 0.05 and less than 0.55;
said fC2 values are greater than or equal to 0.45 and less than 0.65 and said fE2 values are greater than or equal to 0.05 and less than 0.65;
or said fC2 values are greater than or equal to 0.65 and less than 0.75 and said fE2 values are greater than or equal to 0.15 and less than 0.55, when said CBH1 and CBH2 cellobiohydrolases are present at greater than or equal to 75% and less than 85% of the primary cellulases, said fC2 values are greater than or equal to 0.35 and less than 0.45 and said fE2 values are greater than or equal to 0.05 and less than 0.25;
said fC2 values are greater than or equal to 0.45 and less than 0.55 and said fE2 values are greater than or equal to 0.15 and less than 0.65;
or said fC2 values are greater than or equal to 0.55 and less than 0.65 and said fE2 values are greater than or equal to 0.15 and less than 0.35.
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Accused Products
Abstract
An enzyme mixture for the enzymatic hydrolysis of a pretreated lignocellulosic feedstock to soluble sugars is provided. Also provided is a process for hydrolyzing a pretreated lignocellulosic feedstock with an enzyme mixture. The enzymatic hydrolysis comprises adding a cellulase enzyme mixture to a pretreated lignocellulosic feedstock. The cellulase enzyme mixture comprises a primary cellulase mixture of CBH1 and CBH2, and EG1 and EG2. The CBH1 and CBH2 being present at greater than or equal to 55% and less than 85% of the primary cellulase mixture. Furthermore, CBH2 is present at a fraction relative to CBH1 and CBH2, and EG2 is present at a fraction relative to the EG1 and EG2.
43 Citations
23 Claims
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1. A process for enzymatic hydrolysis of a pretreated lignocellulosic feedstock to soluble sugars, the process comprising adding a cellulase enzyme mixture to the pretreated lignocellulosic feedstock, said cellulase enzyme mixture comprising a primary cellulase mixture of CBH1 and CBH2 cellobiohydrolases and EG1 and EG2 endoglucanases, said CBH1 and CBH2 cellobiohydrolases being present at greater than or equal to 55% and less than 85% of the primary cellulase mixture, said CBH2 being present at a fraction relative to CBH1 and CBH2 cellobiohydrolases as defined by fC2 and said EG2 being present at a fraction relative to the EG1 and EG2 endoglucanases as defined by fE2, wherein
when said CBH1 and CBH2 cellobiohydrolases are present at greater than or equal to 55% and less than 65% of the primary cellulases, said fC2 values are greater than or equal to 0.35 and less than 0.45 and said fE2 values are greater than or equal to 0.15 and less than 0.35; -
said fC2 values are greater than or equal to 0.45 and less than 0.55 and said fE2 values greater than or equal to 0.15 and less than 0.25;
orsaid fC2 values are greater than or equal to 0.45 and less than 0.55 and said fE2 values are greater than or equal to 0.35 and less than 0.45, when said CBH1 and CBH2 cellobiohydrolases are present at greater than or equal to 65% and less than 75% of the primary cellulases, said fC2 values are greater than or equal to 0.25 and less than 0.35 and said fE2 values are greater than or equal to 0.05 and less than 0.35;
said fC2 values are greater than or equal to 0.35 and less than 0.45 and said fE2 values are greater than or equal to 0.05 and less than 0.55;
said fC2 values are greater than or equal to 0.45 and less than 0.65 and said fE2 values are greater than or equal to 0.05 and less than 0.65;
orsaid fC2 values are greater than or equal to 0.65 and less than 0.75 and said fE2 values are greater than or equal to 0.15 and less than 0.55, when said CBH1 and CBH2 cellobiohydrolases are present at greater than or equal to 75% and less than 85% of the primary cellulases, said fC2 values are greater than or equal to 0.35 and less than 0.45 and said fE2 values are greater than or equal to 0.05 and less than 0.25;
said fC2 values are greater than or equal to 0.45 and less than 0.55 and said fE2 values are greater than or equal to 0.15 and less than 0.65;
orsaid fC2 values are greater than or equal to 0.55 and less than 0.65 and said fE2 values are greater than or equal to 0.15 and less than 0.35. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A cellulase enzyme mixture comprising a primary cellulase mixture of CBH1 and CBH2 cellobiohydrolases and EG1 and EG2 endoglucanases, said CBH1 and CBH2 cellobiohydrolases being present at greater than or equal to 55% and less than 85% of the primary cellulase mixture, said CBH2 being present at a fraction relative to CBH1 and CBH2 cellobiohydrolases as defined by fC2 and said EG2 being present at a fraction relative to the EG1 and EG2 endoglucanases as defined by fE2, wherein
when said CBH1 and CBH2 cellobiohydrolases are present at greater than or equal to 55% and less than 65% of the primary cellulases, said fC2 values are greater than or equal to 0.35 and less than 0.45 and said fE2 values are greater than or equal to 0.15 and less than 0.35; -
said fC2 values are greater than or equal to 0.45 and less than 0.55 and said fE2 values greater than or equal to 0.15 and less than 0.25;
orsaid fC2 values are greater than or equal to 0.45 and less than 0.55 and said fE2 values are greater than or equal to 0.35 and less than 0.45, when said CBH1 and CBH2 cellobiohydrolases are present at greater than or equal to 65% and less than 75% of the primary cellulases, said fC2 values are greater than or equal to 0.25 and less than 0.35 and said fE2 values are greater than or equal to 0.05 and less than 0.35;
said fC2 values are greater than or equal to 0.35 and less than 0.45 and said fE2 values are greater than or equal to 0.05 and less than 0.55;
said fC2 values are greater than or equal to 0.45 and less than 0.65 and said fE2 values are greater than or equal to 0.05 and less than 0.65;
orsaid fC2 values are greater than or equal to 0.65 and less than 0.75 and said fE2 values are greater than or equal to 0.15 and less than 0.55, when said CBH1 and CBH2 cellobiohydrolases are present at greater than or equal to 75% and less than 85% of the primary cellulases, said fC2 values are greater than or equal to 0.35 and less than 0.45 and said fE2 values are greater than or equal to 0.05 and less than 0.25;
said fC2 values are greater than or equal to 0.45 and less than 0.55 and said fE2 values are greater than or equal to 0.15 and less than 0.65;
orsaid fC2 values are greater than or equal to 0.55 and less than 0.65 and said fE2 values are greater than or equal to 0.15 and less than 0.35. - View Dependent Claims (18, 19, 20, 21, 22, 23)
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Specification